Alpha-mannosidase II Antibody

Novus Biologicals | Catalog # NB100-93414

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Alpha-mannosidase II Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KISSDIKSQNR corresponding to internal region according to NP_002363.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Alpha-mannosidase II Antibody

Western Blot: Alpha-mannosidase II Antibody [NB100-93414]

Western Blot: Alpha-mannosidase II Antibody [NB100-93414]

Western Blot: Alpha-mannosidase II Antibody [NB100-93414] - HEK293 overexpressing Man2A1 and probed with this antibody (mock transfection in first lane).
Western Blot: Alpha-mannosidase II Antibody [NB100-93414]

Western Blot: Alpha-mannosidase II Antibody [NB100-93414]

Western Blot: Alpha-mannosidase II Antibody [NB100-93414] - Analysis of alpha-mannosidase II antibody [NB100-93414] (0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Alpha-mannosidase II Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 150 kDa band observed in human placenta lysates (calculated MW of 131 kDa band according to NP_002363.2). In transfected HEK293 transiently expressing MAN2A1 a band of approx. 98 kDa is observed. This band is not observed in the non-transfected HEK293. An anonymous customer has reported positive results on human dermal fibroblasts.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Alpha-mannosidase II

This gene encodes a protein which is a member of family 38 of the glycosyl hydrolases. The protein is located in the Golgi and catalyzes the final hydrolytic step in the asparagine-linked oligosaccharide (N-glycan) maturation pathway. Mutations in the mouse homolog of this gene have been shown to cause a systemic autoimmune disease similar to human systemic lupus erythematosus. [provided by RefSeq]

Alternate Names

alpha-mannosidase 2, AMan II, EC 3.2.1, EC 3.2.1.114, Golgi alpha-mannosidase II, golgi integral membrane protein 7, GOLIM7, Man II, MANA2MANII, Mannosidase alpha class 2A member 1, mannosidase, alpha type II, mannosidase, alpha, class 2A, member 1, Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

Entrez Gene IDs

4124 (Human)

Gene Symbol

MAN2A1

UniProt

Q16706 (Human)

Additional Alpha-mannosidase II Products

Product Documents for Alpha-mannosidase II Antibody

Certificate of Analysis

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Product Specific Notices for Alpha-mannosidase II Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Alpha-mannosidase II Antibody

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