AMICA/JAML Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-14286
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Western Blot: AMICA/JAML Antibody [NBP2-14286]
Western Blot: AMICA/JAML Antibody [NBP2-14286] - Analysis in human lung tissue.Applications for AMICA/JAML Antibody - BSA Free
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Read 1 review rated 5 using NBP2-14286 in the following applications:
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Background: AMICA/JAML
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Product Specific Notices for AMICA/JAML Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Application: Immunohistochemistry-ParaffinSample Tested: kidneySpecies: MouseVerified Customer | Posted 12/15/2016Antibody Storage Conditions:Store at 4C short term. Aliquot and store at -20℃ long term. Avoid freeze-thaw cycles. Test Sample Information: Species & Treatments:mouse Tissue: kidney Fixative Composition: 4% Paraformaldehyde solution Fixation Time & Temperature: 24h,4℃ Tissue Processing: paraffin embedding Antigen Retrieval Details (for IHC-P): Method & Buffer Used: Citric Acid Antigen Retrieval Buffer Time & Temperature: >95℃ 20min Blocking Procedure: Blocking Solution: 5% Goat serum,0.1%BSA Time & Temperature: 1h,room temperature Endogenous Peroxidase Blocking (For IHC-P only) H2O2 cocktail composition: 3% H2O2 Blocking Time: 10min Primary Antibody Dilution: 1:100 Diluent Buffer: 0.1%BSA(PBS) Time & Temperature: overnight,4℃ Washing Conditions: Wash Buffer Composition: PBS Method (Immersion, Rinsing etc.): Immersion Times/Washing: 5min Repetitions: 3 Secondary Antibody Manufacturer and Catalog #: Zhongshan golden bridge ZB-2301 Secondary description:goat anti-rabbit Dilution: 1:100 Diluent Buffer: PBS Incubation Time & Temperature: 1h,room temperature Post-Secondary Washing: 5min X 3 times Detection Method: Detection: Zhongshan golden bridge SP-9000 Procedure: according to the instruction book DAB-Incubation Time (if applicable):3min Controls: Negative Control: Control rabbit IgG
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