Amiloride hydrochloride
Tocris Bioscience | Catalog # 0890
Key Product Details
Description
Product Description
Amiloride hydrochloride is a Na+ channel blocker. Defines the I2A-amiloride sensitive and I2B-amiloride insensitive imidazoline binding Blocks TRPP3, acid sensing- (ASIC) and mechanogated membrane-ion channels, as well as the Na+/H+ exchanger. Also inhibits urokinase-type plasminogen activator (uPA); has no effect on tissue-type plasminogen activator.
Product Specifications for Amiloride hydrochloride
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 1.33 | 5 with gentle warming | |
| DMSO | 26.61 | 100 |
Preparing Stock Solutions for Amiloride hydrochloride
The following data is based on the product molecular weight 266.09.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.76 mL | 18.79 mL | 37.58 mL |
| 5 mM | 0.75 mL | 3.76 mL | 7.52 mL |
| 10 mM | 0.38 mL | 1.88 mL | 3.76 mL |
| 50 mM | 0.08 mL | 0.38 mL | 0.75 mL |
Calculators
Background References
References are publications that support the biological activity of the product. See our Citations tab to view 22 publications citing the usage of this product.
- Jetti Evaluation of the role of nitric oxide in acid sensing ion channel mediated cell death. Nitric Oxide 2010 PMID: 20045740
- Ernsberger A second generation of centrally acting antihypertensive agents act on putative I1-imidazoline receptors. J.Cardiovasc.Pharmacol.
- Hamill and McBride The pharmacology of mechanogated membrane ion channels. Pharmacol.Rev. 1996 PMID: 8804105
- Kleyman Amiloride and its analogues as tools in the study of ion transport. J.Membr.Biol. 1988 PMID: 2852254
- Dai Inhibition of TRPP3 channel by amil. and analogs. Mol.Pharmacol. 2007 PMID: 17804601
Product Documents for Amiloride hydrochloride
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Amiloride hydrochloride
For research use only
Related Research Areas
Citations for Amiloride hydrochloride
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Species: CanineAssay Type: In VitroVerified Customer | Posted 03/01/2019Dissolve the powder in 100 mM DMSO, and dilute according to minimize vehicle effects.Amiloride was combined with chemotherapy to assess its potential to kill canine osteosarcoma cells, and was found to be synergistic with doxorubicin chemotherapy. It was also used by the Seahorse analyzer to show that the extracellular acidification rate (ECAR) decreases after amiloride exposure.
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Species: MouseAssay Type: In VivoCell Line/Tissue: acute brain sliceVerified Customer | Posted 01/10/2018Used to show ASIC channels were not involved in the studied effect. No effect was had, as expected, however it's hard to control for the drug efficacy as we did not show ASIC blockade directly.
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