Chemical Name: 3,5-Diamino-N-(aminoiminomethyl)-6-chloropyrazinecarboxamide hydrochloride
Biological ActivityNa+ channel blocker. Defines the I2A-amiloride sensitive and I2B-amiloride insensitive imidazoline binding Blocks TRPP3, acid sensing- (ASIC) and mechanogated membrane-ion channels, as well as the Na+/H+ exchanger. Also inhibits urokinase-type plasminogen activator (uPA); has no effect on tissue-type plasminogen activator.
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Tocris products are intended for laboratory research use only, unless stated otherwise.
Evaluation of the role of nitric oxide in acid sensing ion channel mediated cell death.
Jetti et al.
Nitric Oxide, 2010;22:213
The pharmacology of mechanogated membrane ion channels.
Hamill and McBride
Amiloride and its analogues as tools in the study of ion transport.
Kleyman et al.
A second generation of centrally acting antihypertensive agents act on putative I1-imidazoline receptors.
Ernsberger et al.
Inhibition of TRPP3 channel by amil. and analogs.
Dai et al.
Citations for Amiloride hydrochloride
The citations listed below are publications that use Tocris products. Selected citations for Amiloride hydrochloride include:
7 Citations: Showing 1 - 7
NCX1 Represents an Ionic Na+ Sensing Mechanism in Macrophages
Authors: Neubert Et al.
PloS Biol 2020;18
The Role of the Anion in Salt (NaCl) Detection by Mouse Taste Buds.
Authors: Roebber Et al.
J Neurosci 2019;39:6224
Osmotic induction of cyclooxygenase-2 in RPE cells: Stimulation of inflammasome activation.
Authors: Messerschmidt Et al.
Mol Vis 2019;25:329
Effects of the potassium-sparing diuretic amil. on chemotherapy response in canine osteosarcoma cells.
Authors: Poon Et al.
J Vet Intern Med 2018;33:800
Dendritic cell amiloride-sensitive channels mediate sodium-induced inflammation and hypertension.
Cell Rep 2017;21(4):1009
Polycaprolactone/maltodextrin nanocarrier for intracellular drug delivery: formulation, uptake mechanism, internalization kinetics, and subcellular localization.
Authors: Korang-Yeboah Et al.
J Neurosci 2015;10:4763
Antinociceptive effects of amil. and benzamil in neuropathic pain model rats.
Authors: Jeong Et al.
Int J Nanomedicine 2013;28:1238
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Reviews for Amiloride hydrochloride
Average Rating: 4 (Based on 2 Reviews)
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Amiloride was combined with chemotherapy to assess its potential to kill canine osteosarcoma cells, and was found to be synergistic with doxorubicin chemotherapy. It was also used by the Seahorse analyzer to show that the extracellular acidification rate (ECAR) decreases after amiloride exposure.
Used to show ASIC channels were not involved in the studied effect. No effect was had, as expected, however it's hard to control for the drug efficacy as we did not show ASIC blockade directly.