Aminopeptidase N/CD13 Antibody (WM15) [FITC]

Novus Biologicals | Catalog # NB110-55358

Clone WM15 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Human, Primate

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # WM15
View all Aminopeptidase N/CD13 Primary Antibodies »

Product Specifications

Immunogen

Human AML cells (NM_001150.2).

Specificity

The antibody WM15 recognises the human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors. HLDA III; WS Code M 213 HLDA IV; WS Code M 44 HLDA IV; WS Code M 209 HLDA V; WS Code M MA191

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aminopeptidase N/CD13 Antibody (WM15) [FITC]

Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [FITC] [NB110-55358] - Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) PerCP.
Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [FITC] [NB110-55358] - Surface staining of human peripheral blood leukocytes with anti-CD13.
Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [FITC] [NB110-55358] - Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) PE-DyLight 594.
Flow Cytometry: Aminopeptidase N/CD13 Antibody (WM15) [FITC] [NB110-55358] - Surface staining of human peripheral blood leukocytes with anti-CD13 (WM15) biotin, streptavidin-APC.

Applications for Aminopeptidase N/CD13 Antibody (WM15) [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

Phosphate buffered saline (PBS), pH 7.4

Preservative

15mM Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Aminopeptidase N/CD13

CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.

Alternate Names

Aminopeptidase M, ANPEP, APN, CD13, gp150, PEPN

Gene Symbol

ANPEP

Additional Aminopeptidase N/CD13 Products

Product Documents for Aminopeptidase N/CD13 Antibody (WM15) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Aminopeptidase N/CD13 Antibody (WM15) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Aminopeptidase N/CD13 Antibody (WM15) [FITC]

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  • Q: Our customer would like to know the recommended titer to start flow cytometry with NB110-55358. Would you please help suggest? The customer also noticed that the concentration of this antibody is described as "Predilute mg/ml." We wonder what it means. If the concentration is different from lot to lot. Would you please help inform the conc. of the latest lot?

    A: The FITC-conjugated CD13 antibody with catalogue number NB110-55358 can be utilised for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. That the concentration is listed as predilute mg/ml simply means that the product is ready for direct use.

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