Recombinant Human AMPK gamma 1 His Protein

Name: Recombinant Human AMPK gamma 1 His Protein
Brand: Novus Biologicals
SKU: NBP2-23377
Price: 329 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-23377

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all AMPK gamma 1 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-331 of Human AMPK gamma 1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMETVISS DSSPAVENEH PQETPESNNS VYTSFMKSHR CYDLIPTSSK LVVFDTSLQV KKAFFALVTN GVRAAPLWDS KKQSFVGMLT ITDFINILHR YYKSALVQIY ELEEHKIETW REVYLQDSFK PLVCISPNAS LFDAVSSLIR NKIHRLPVID PESGNTLYIL THKRILKFLK LFITEFPKPE FMSKSLEELQ IGTYANIAMV RTTTPVYVAL GIFVQHRVSA LPVVDEKGRV VDIYSKFDVI NLAAEKTYNN LDVSVTKALQ HRSHYFEGVL KCYLHETLET IINRLVEAEV HRLVVVDEND VVKGIVSLSD ILQALVLTGG EKKP

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human AMPK gamma 1 His Protein

SDS-PAGE: Recombinant Human AMPK gamma 1 His Protein [NBP2-23377]

SDS-Page: Recombinant Human AMPK gamma 1 Protein [NBP2-23377]

Formulation, Preparation, and Storage

NBP2-23377

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AMPK gamma 1

5'-AMP-activated protein kinase subunit gamma-1 isoform 1, also known as PRKAG1, is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. Recombinant human PRKAG1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

AMP-activated Protein Kinase gamma 1

Alternate Names

AMPKG, AMPKG1, PRKAG1

Gene Symbol

PRKAG1

Additional AMPK gamma 1 Products

Product Documents for Recombinant Human AMPK gamma 1 His Protein

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Product Specific Notices for Recombinant Human AMPK gamma 1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy

Intracellular Kinases

Obesity

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