Anillin Antibody (CL0301) - BSA Free

Novus Biologicals | Catalog # NBP2-52907

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0301

Format

BSA Free
View all Anillin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q9NQW6, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Anillin Antibody (CL0301) - BSA Free

Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0301) [NBP2-52907]

Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0301) [NBP2-52907]

Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0301) [NBP2-52907] - Staining of U-251 cells using the Anti-ATAD2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining in human testis and skeletal muscle tissues. Corresponding ANLN RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0301) [NBP2-52907]

Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0301) [NBP2-52907]

Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0301) [NBP2-52907] - Staining of A-431 cells using the Anti-ATAD2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining of human lung cancer shows moderate to strong nuclear positivity in a subset of tumor cells.
Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining of human skin shows moderate to strong nuclear positivity in a subset of dermal cells.
Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining of human rectum shows moderate to strong nuclear positivity in a subset of glandular cells.
Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.

Applications for Anillin Antibody (CL0301) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Anillin

Anillin is a 124 kDa scaffolding protein that plays an important role in cell division. Anillin interacts with septins and mediates septin filament assembly and stabilization in the construction of the contractile ring responsible for cleavage furrow formation and cytokinesis.

Alternate Names

actin-binding protein anillin, ANILLIN, anillin (Drosophila Scraps homolog), actin binding protein, anillin, actin binding protein, anillin, actin binding protein (scraps homolog, Drosophila), DKFZp779A055, Drosophila Scraps homolog, scra, Scraps

Gene Symbol

ANLN

Additional Anillin Products

Product Documents for Anillin Antibody (CL0301) - BSA Free

Certificate of Analysis

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Product Specific Notices for Anillin Antibody (CL0301) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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