Anillin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-38630

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Anillin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: DLFSDVLEEGELDMEKSQEEMDQALAESSEEQEDALNISSMSLLAPLAQTVGVVSPESLVSTPRLELKDTSRSDESPKPGKFQRTRVPRAESGDSLGSEDRDLLYSIDAYRSQRFKETERPSIKQVIVRKEDV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Anillin Antibody - BSA Free

Western Blot: Anillin Antibody [NBP2-38630]

Western Blot: Anillin Antibody [NBP2-38630]

Western Blot: Anillin Antibody [NBP2-38630] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-ANLN antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Anillin Antibody [NBP2-38630]

Immunocytochemistry/ Immunofluorescence: Anillin Antibody [NBP2-38630]

Immunocytochemistry/Immunofluorescence: Anillin Antibody [NBP2-38630] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630]

Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630]

Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630]

Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630]

Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630]

Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630]

Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Analysis in human testis and skeletal muscle tissues. Corresponding ANLN RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630]

Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630]

Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Staining of human colon shows moderate to strong nuclear positivity in a subset of glandular cells.
Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630]

Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630]

Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Staining of human placenta shows moderate to strong nuclear positivity in a subset of trophoblastic cells.
Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630]

Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630]

Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for Anillin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Anillin

Anillin is a 124 kDa scaffolding protein that plays an important role in cell division. Anillin interacts with septins and mediates septin filament assembly and stabilization in the construction of the contractile ring responsible for cleavage furrow formation and cytokinesis.

Alternate Names

actin-binding protein anillin, ANILLIN, anillin (Drosophila Scraps homolog), actin binding protein, anillin, actin binding protein, anillin, actin binding protein (scraps homolog, Drosophila), DKFZp779A055, Drosophila Scraps homolog, scra, Scraps

Gene Symbol

ANLN

UniProt

Q9NQW6 (Human)

Additional Anillin Products

Product Documents for Anillin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Anillin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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