Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ANKRD17 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 775 and 825 of human ankyrin repeat domain 17 using the numbering given in entry NP_115593.3 (GeneID 26057).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

274 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ANKRD17 Antibody - BSA Free

Western Blot: ANKRD17 Antibody [NB100-86995]

Western Blot: ANKRD17 Antibody [NB100-86995]

Western Blot: ANKRD17 Antibody [NB100-86995] - Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ANKRD17 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: ANKRD17 Antibody [NB100-86995]

Immunoprecipitation: ANKRD17 Antibody [NB100-86995]

Immunoprecipitation: ANKRD17 Antibody [NB100-86995] - Detection of human ANKRD17 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ANKRD17 antibody NB100-86995 used for IP at 3 ug per reaction. ANKRD17 was also immunoprecipitated by rabbit anti-ANKRD17 antibody NB100-86996. For blotting immunoprecipitated ANKRD17, NB100-86995 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for ANKRD17 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 mcg/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ANKRD17

This gene encodes a protein with ankyrin repeats, which are associated with protein-protein interactions. Studies in mice suggest that this protein is involved in liver development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

ankyrin repeat domain 17, ankyrin repeat domain-containing protein 17, Gene trap ankyrin repeat protein, GTARSerologically defined breast cancer antigen NY-BR-16, KIAA0697FLJ22206, NY-BR-16

Entrez Gene IDs

26057 (Human)

Gene Symbol

ANKRD17

UniProt

O75179 (Human)

Additional ANKRD17 Products

Product Documents for ANKRD17 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ANKRD17 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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