ANKRD27 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-50030

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ANKRD27 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1000 and 1050 of human Vps9 Domain and Ankyrin-Repeat-Containing Protein using the numbering given in entry NP_115515.2 (GeneID 84079).

Reactivity Notes

Orangutan (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ANKRD27 Antibody - BSA Free

Western Blot: ANKRD27 Antibody [NBP1-50030]

Western Blot: ANKRD27 Antibody [NBP1-50030]

Western Blot: ANKRD27 Antibody [NBP1-50030] - Detection of Human Varp by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Varp antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Varp was also immunoprecipitated by rabbit anti-Varp antibody which recognizes an upstream epitope. For blotting immunoprecipitated Varp, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for ANKRD27 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ANKRD27

ANKRD27 may be a Rab21 guanine exchange factor and regulate endosome dynamics

Alternate Names

ankyrin repeat domain 27 (VPS9 domain), ankyrin repeat domain-containing protein 27, DKFZp434L0718, FLJ00040, VARP, Vps9 domain and ankyrin-repeat-containing protein, VPS9 domain-containing protein, VPS9-ankyrin-repeat protein

Gene Symbol

ANKRD27

Additional ANKRD27 Products

Product Documents for ANKRD27 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ANKRD27 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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