Annexin V Antibody (M017)

Novus Biologicals | Catalog # NBP3-23207

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M017
View all Annexin V Primary Antibodies »

Product Specifications

Immunogen

Clone M017 was generated from a proprietary antigen related to human Annexin V in the MDA-MB-231 breast cancer cell line. [UniProt# P08758]

Specificity

Clone M017 detects a 35 kDa protein corresponding to the molecular mass of Annexin V on SDS-PAGE immunoblots of human A431, MDA-MB-231, MeWo, and PC3 cells, as well as human skin, breast, lung, and brain. Mass spectrometry analysis of immunoprecipitates using NBP3-23207 in human MDA-MB-231 cell lysates confirms this antibody only detects Annexin A5 protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Annexin V Antibody (M017)

Annexin V Antibody (M017)

Western Blot: Annexin V Antibody (M017) [NBP3-23207] -

Western Blot: Annexin V Antibody (M017) [NBP3-23207] - Western blot of human PC3 cells (lane 1), human breast tissue (lane 2), lung tissue (lane 3), skin tissue (lane 4), and brain tissue (lane 5). The blot was probed with mouse monoclonal anti-Annexin V antibody (NBP3-23207) at 1:500 (lanes 1-5).
Annexin V Antibody (M017)

Western Blot: Annexin V Antibody (M017) [NBP3-23207] -

Western Blot: Annexin V Antibody (M017) [NBP3-23207] - Western blot of A431 cell lysate only (lane 1), NBP3-23207 antibody only (lane 2), and NBP3-23207 antibody immunoprecipitate from A431 (lane 3). The blot was probed with mouse monoclonal anti-Annexin V antibody (NBP3-23207) at 1:500 (lanes 1-3). The asterisk shows the antibody heavy chain in immunopreciptates at 50 kDa, while Annexin V band is observed at 35 kDa.

Applications for Annexin V Antibody (M017)

Application
Recommended Usage

Immunoprecipitation

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Annexin V

Annexin V is encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

Alternate Names

ANX5, ANXA5, ANXV

Gene Symbol

ANXA5

Additional Annexin V Products

Product Documents for Annexin V Antibody (M017)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Annexin V Antibody (M017)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Coagulation

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FAQs for Annexin V Antibody (M017)

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?

    A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).

  • Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?

    A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.

  • Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?

    A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).

  • Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?

    A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.

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