Human Annexin V ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP1-91253
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Key Product Details
Sample Type & Volume Required Per Well
Sensitivity
Assay Range
Product Specifications
Assay Type
Kit Type
Species
Specificity
Description
Precision
Intra-Assay Precision (Precision within an assay) CV% 10%
Inter-Assay Precision (Precision between assays) CV% 11.00%
Recovery for Human Annexin V ELISA Kit (Colorimetric)
Recovery
Scientific Data Images for Human Annexin V ELISA Kit (Colorimetric)
ELISA: Human Annexin V ELISA Kit (Colorimetric) [NBP1-91253]
ELISA: Annexin V ELISA Kit [NBP1-91253] - Standard curveKit Contents for Human Annexin V ELISA Kit (Colorimetric)
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Annexin V
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Biotin-Conjugate anti-human Annexin V monoclonal antibody
- Human Annexin V Standard lyophilized, 100 ng/ml upon reconstitution
- Sample Diluent
- Stop Solution (1M Phosphoric acid)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Preparation and Storage
Shipping
Stability & Storage
Background: Annexin V
Alternate Names
Gene Symbol
Additional Annexin V Products
Product Documents for Human Annexin V ELISA Kit (Colorimetric)
Certificate of Analysis
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Product Specific Notices for Human Annexin V ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Human Annexin V ELISA Kit (Colorimetric)
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Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?
A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).
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Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?
A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.
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Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?
A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).
-
Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?
A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.