Recombinant Mouse Annexin V His Protein
Novus Biologicals | Catalog # NBP2-52063
![SDS-PAGE: Recombinant Mouse Annexin V His Protein [NBP2-52063]](https://resources.rndsystems.com/images/products/Annexin-V-Recombinant-Protein-SDS-Page-NBP2-52063-img0001.jpg "SDS-PAGE: Recombinant Mouse Annexin V His Protein [NBP2-52063]")
Key Product Details
Source
Tag
Applications
Product Specifications
Description
Source: E.coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMATRGTV TDFPGFDGRA DAEVLRKAMK GLGTDEDSIL NLLTSRSNAQ RQEIAQEFKT LFGRDLVDDL KSELTGKFEK LIVAMMKPSR LYDAYELKHA LKGAGTDEKV LTEIIASRTP EELSAIKQVY EEEYGSNLED DVVGDTSGYY QRMLVVLLQA NRDPDTAIDD AQVELDAQAL FQAGELKWGT DEEKFITIFG TRSVSHLRRV FDKYMTISGF QIEETIDRET SGNLEQLLLA VVKSIRSIPA YLAETLYYAM KGAGTDDHTL IRVVVSRSEI DLFNIRKEFR KNFATSLYSM IKGDTSGDYK KALLLLCGGE DD
Purity
Predicted Molecular Mass
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Protein / Peptide Type
Scientific Data Images for Recombinant Mouse Annexin V His Protein
SDS-PAGE: Recombinant Mouse Annexin V His Protein [NBP2-52063]
SDS-Page: Recombinant Mouse Annexin V Protein [NBP2-52063] - 15% SDS Page (3 ug)Formulation, Preparation, and Storage
NBP2-52063
| Formulation | PBS (pH 7.4), 10% glycerol, 1 mM DTT |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: Annexin V
Alternate Names
Gene Symbol
Additional Annexin V Products
Product Documents for Recombinant Mouse Annexin V His Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Recombinant Mouse Annexin V His Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Related Research Areas
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FAQs for Recombinant Mouse Annexin V His Protein
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Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?
A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).
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Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?
A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.
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Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?
A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).
-
Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?
A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.