Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ANP32A Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human ANP32A. The immunogen is located within the last 50 amino acids of ANP32A.

Reactivity Notes

This sequence is identical between human and rat PHAP I.

Specificity

This polyclonal antibody has no cross-reaction to PHAP I2a and PHAP III.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ANP32A Antibody - BSA Free

Western Blot: ANP32A AntibodyBSA Free [NBP2-25097]

Western Blot: ANP32A AntibodyBSA Free [NBP2-25097]

Western Blot: ANP32A Antibody [NBP2-25097] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: ANP32A NBP2-25097 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/Immunofluorescence: ANP32A Antibody [NBP2-25097] -

Immunocytochemistry/Immunofluorescence: ANP32A Antibody [NBP2-25097] - Raji cells with PHAP I antibody at 2 ug/mL.
Western Blot: ANP32A AntibodyBSA Free [NBP2-25097]

Western Blot: ANP32A AntibodyBSA Free [NBP2-25097]

Western Blot: ANP32A Antibody [NBP2-25097] - Western blot analysis of PHAP I expression in human Raji cell lysate with PHAP antibody I at 2 ug/mL (lane A) and 4 ug/mL (lane B), respectively.
Western Blot: ANP32A AntibodyBSA Free [NBP2-25097]

Western Blot: ANP32A AntibodyBSA Free [NBP2-25097]

Western Blot: ANP32A Antibody [NBP2-25097] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: ANP32A NBP2-25097 (2 ug/mL), ANP32A NBP2-81794 (1 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
ANP32A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ANP32A Antibody - BSA Free [NBP2-25097] -

Immunocytochemistry/ Immunofluorescence: ANP32A Antibody - BSA Free [NBP2-25097] - Validation of PHAP I in Raji Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Raji Cells labeling PHAP I with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Applications for ANP32A Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2-10 ug/mL

Western Blot

2-4 ug/mL

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ANP32A

PHAP I Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.

Alternate Names

acidic (leucine-rich) nuclear phosphoprotein 32 family, member A, acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32, cerebellar leucine rich acidic nuclear protein, I1PP2A, inhibitor-1 of protein phosphatase-2A, LANPPutative HLA-DR-associated protein I, Leucine-rich acidic nuclear protein, MAPMC15orf1, mapmodulin, PHAP1MGC150373, PHAPIMGC119787, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, PP32, putative human HLA class II associated protein I

Gene Symbol

ANP32A

UniProt

P39687 (Human)

Additional ANP32A Products

Product Documents for ANP32A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ANP32A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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