PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.
Anti-PAR Polyclonal Antibody (Rabbit)
R&D Systems | Catalog # 4336-BPC-100
Discontinued Product
4336-BPC-100 has been discontinued.
An alternative/replacement product is available:
4335-MC-100. View all PAR/pADPr products.
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Human, Mouse, Rat, Avian - Chicken, Drosophila, Insect - Drosophila melanogaster, Xenograft
Applications
Validated:
Western Blot, ELISA
Cited:
Immunohistochemistry, Western Blot, Immunocytochemistry, Immunoprecipitation, Bioassay, ELISA Detection
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Applications for Anti-PAR Polyclonal Antibody (Rabbit)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: PAR/pADPr
Long Name
Poly [ADP-ribose] Polymer
Alternate Names
PAR
Additional PAR/pADPr Products
Product Documents for Anti-PAR Polyclonal Antibody (Rabbit)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Anti-PAR Polyclonal Antibody (Rabbit)
For research use only
Related Research Areas
Citations for Anti-PAR Polyclonal Antibody (Rabbit)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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