PAR/pADPr Antibody

Catalog # Availability Size / Price Qty
4335-MC-100
PAR/pADPr Antibody in Data
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Product Details
Citations (22)
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PAR/pADPr Antibody Summary

Specificity
The antibody is specific for PAR polymers 2 to 50 units long, but does not recognize structurally related RNA, DNA, ADP-ribose monomers, NAD, or other nucleic acid monomers.
Source
Monoclonal Mouse IgG3 Clone # 10HA
Purification
Protein A or G purified from ascites
Immunogen
Purified ADP-ribose polymers between 2 and 50 units long
Formulation
Supplied as a solution in TBS containing Glycerol.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
Jurkat human acute T cell leukemia cell line treated with H2O2
ELISA
As determined by testing.
 
Immunoprecipitation
As determined by testing.
 
Immunoaffinity Purification
As determined by testing.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Jurkat human acute T cell leukemia cell line treated with 1 mM H2O2 for 5 minutes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot View Larger

Detection of Human PAR/pADPr by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 2 mM H2O2 for 5 minutes. PVDF membrane was probed with 1:1000 dilution of Mouse Anti-PAR/pADPr Monoclonal Antibody (Catalog # 4335-MC-100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). Specific bands were detected for PAR polymers, as indicated. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Intracellular Staining by Flow Cytometry View Larger

Detection of PAR in Jurkat cells by Flow Cytometry. Jurkat human acute T cell leukemia cell line treated with 1 mM H2O2 for 5 minutes (filled histogram) or resting (open histogram) were stained with Mouse Anti-PAR Monoclonal Antibody (Catalog # 4335-MC-100) followed by Goat anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed and permeabilized using FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.

Reconstitution Calculator

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Preparation and Storage

Reconstitution


Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: PAR/pADPr

PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.

Long Name
Poly [ADP-ribose] Polymer
Alternate Names
pADPr; PAR; Poly(ADP-ribose)

Product Datasheets

Citations for PAR/pADPr Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. MTH1 and OGG1 maintain a low level of 8-oxoguanine in Alzheimer's brain, and prevent the progression of Alzheimer's pathogenesis
    Authors: S Oka, J Leon, K Sakumi, N Abolhassan, Z Sheng, D Tsuchimoto, FM LaFerla, Y Nakabeppu
    Scientific Reports, 2021;11(1):5819.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Selective modulation by PARP-1 of HIF-1&alpha-recruitment to chromatin during hypoxia is required for tumor adaptation to hypoxic conditions
    Authors: JM Martí, A Garcia-Dia, D Delgado-Be, F O'Valle, A González-F, O Carlevaris, JM Rodríguez-, JC Amé, F Dantzer, GL King, K Dziedzic, E Berra, E de Álava, AT Amaral, EM Hammond, FJ Oliver
    Redox Biology, 2021;41(0):101885.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Clinical PARP inhibitors do not abrogate PARP1 exchange at DNA damage sites in vivo
    Authors: Z Shao, BJ Lee, É Rouleau-Tu, MF Langelier, X Lin, VM Estes, JM Pascal, S Zha
    Nucleic Acids Res., 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  4. Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) with OT-82 induces DNA damage, cell death, and suppression of tumor growth in preclinical models of Ewing sarcoma
    Authors: AE Gibson, C Yeung, SH Issaq, VJ Collins, M Gouzoulis, Y Zhang, J Ji, A Mendoza, CM Heske
    Oncogenesis, 2020;9(9):80.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Functional Assay
  5. Poly(ADP-ribose) Polymerase 1 (PARP1) restrains MyoD-dependent gene expression during muscle differentiation
    Authors: F Matteini, O Andresini, S Petrai, C Battistell, MN Rossi, R Maione
    Sci Rep, 2020;10(1):15086.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  6. XRN2 interactome reveals its synthetic lethal relationship with PARP1 inhibition
    Authors: PL Patidar, T Viera, JC Morales, N Singh, EA Motea, M Khandelwal, FJ Fattah
    Sci Rep, 2020;10(1):14253.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Pathogenic ARH3 mutations result in ADP-ribose chromatin scars during DNA strand break repair
    Authors: H Hanzlikova, E Prokhorova, K Krejcikova, Z Cihlarova, I Kalasova, J Kubovciak, J Sachova, R Hailstone, J Brazina, S Ghosh, S Cirak, JG Gleeson, I Ahel, KW Caldecott
    Nat Commun, 2020;11(1):3391.
  8. Crosstalk between hydroxytyrosol, a major olive oil phenol, and HIF-1 in MCF-7 breast cancer cells
    Authors: J Calahorra, E Martínez-L, JM Granadino-, JM Martí, A Cañuelo, S Blanco, FJ Oliver, E Siles
    Sci Rep, 2020;10(1):6361.
    Species: Human
    Sample Types: CCS MCF-7 cell line
  9. Poly(ADP-ribosyl)ation mediates early phase histone eviction at DNA lesions
    Authors: G Yang, Y Chen, J Wu, SH Chen, X Liu, AK Singh, X Yu
    Nucleic Acids Res., 2020;0(0):.
    Species: Human
    Sample Types: Cell Lysate
    Applications: Immunoprecipitation
  10. Tankyrase disrupts metabolic homeostasis and promotes tumorigenesis by inhibiting LKB1-AMPK signalling
    Authors: N Li, Y Wang, S Neri, Y Zhen, LWR Fong, Y Qiao, X Li, Z Chen, C Stephan, W Deng, R Ye, W Jiang, S Zhang, Y Yu, MC Hung, J Chen, SH Lin
    Nat Commun, 2019;10(1):4363.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  11. MORC2 regulates DNA damage response through a PARP1-dependent pathway
    Authors: L Zhang, DQ Li
    Nucleic Acids Res., 2019;47(16):8502-8520.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. A PARP1-BRG1-SIRT1 axis promotes HR repair by reducing nucleosome density at DNA damage sites
    Authors: Y Chen, H Zhang, Z Xu, H Tang, A Geng, B Cai, T Su, J Shi, C Jiang, X Tian, A Seluanov, J Huang, X Wan, Y Jiang, V Gorbunova, Z Mao
    Nucleic Acids Res., 2019;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Poly (ADP) ribose glycohydrolase can be effectively targeted in pancreatic cancer
    Authors: A Jain, LC Agostini, GA McCarthy, SN Chand, A Ramirez, A Nevler, J Cozzitorto, CW Schultz, CY Lowder, KM Smith, ID Waddell, M Raitses-Gu, C Stossel, YG Gorman, D Atias, CJ Yeo, JM Winter, KP Olive, T Golan, MJ Pishvaian, D Ogilvie, DI James, AM Jordan, JR Brody
    Cancer Res., 2019;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Overexpressed ABCB1 Induces Olaparib-Taxane Cross-Resistance in Advanced Prostate Cancer
    Authors: AP Lombard, C Liu, CM Armstrong, LS D'Abronzo, W Lou, H Chen, M Dall'Era, PM Ghosh, CP Evans, AC Gao
    Transl Oncol, 2019;12(7):871-878.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. The coronavirus macrodomain is required to prevent PARP-mediated inhibition of virus replication and enhancement of IFN expression
    Authors: ME Grunewald, Y Chen, C Kuny, T Maejima, R Lease, D Ferraris, M Aikawa, CS Sullivan, S Perlman, AR Fehr
    PLoS Pathog., 2019;15(5):e1007756.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Poly(ADP-ribose) polymerase 1 accelerates vascular calcification by upregulating Runx2
    Authors: C Wang, W Xu, J An, M Liang, Y Li, F Zhang, Q Tong, K Huang
    Nat Commun, 2019;10(1):1203.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. NADP+ is an endogenous PARP inhibitor in DNA damage response and tumor suppression
    Authors: C Bian, C Zhang, T Luo, A Vyas, SH Chen, C Liu, MA Kassab, Y Yang, M Kong, X Yu
    Nat Commun, 2019;10(1):693.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Mono-ADP-ribosylation of H3R117 traps 5mC hydroxylase TET1 to impair demethylation of tumor suppressor gene TFPI2
    Authors: M Li, Y Tang, Q Li, M Xiao, Y Yang, Y Wang
    Oncogene, 2019;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  19. Tripartite Motif-containing 33 (TRIM33) protein functions in the poly(ADP-ribose) polymerase (PARP)-dependent DNA damage response through interaction with Amplified in Liver Cancer 1 (ALC1) protein.
    Authors: Kulkarni A, Oza J, Yao M, Sohail H, Ginjala V, Tomas-Loba A, Horejsi Z, Tan A, Boulton S, Ganesan S
    J Biol Chem, 2013;288(45):32357-69.
  20. BAL1 and its partner E3 ligase, BBAP, link Poly(ADP-ribose) activation, ubiquitylation, and double-strand DNA repair independent of ATM, MDC1, and RNF8.
    Authors: Yan Q, Xu R, Zhu L, Cheng X, Wang Z, Manis J, Shipp M
    Mol Cell Biol, 0;33(4):845-57.
  21. The PARP3- and ATM-dependent phosphorylation of APLF facilitates DNA double-strand break repair.
    Authors: Fenton A, Shirodkar P, Macrae C, Meng L, Koch C
    Nucleic Acids Res, 0;41(7):4080-92.
  22. Loss of 53BP1 causes PARP inhibitor resistance in Brca1-mutated mouse mammary tumors.
    Authors: Jaspers J, Kersbergen A, Boon U, Sol W, van Deemter L, Zander S, Drost R, Wientjens E, Ji J, Aly A, Doroshow J, Cranston A, Martin N, Lau A, O'Connor M, Ganesan S, Borst P, Jonkers J, Rottenberg S
    Cancer Discov, 0;3(1):68-81.

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