Human APAF-1 ELISA Kit (Colorimetric)

Name: Human APAF-1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-68232
Price: 709 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-68232

Submit a review

Product Documents

100% Guarantee

Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

View all APAF-1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human APAF1 in samples. No significant cross-reactivity or interference between Human APAF1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.18%

Inter-Assay Precision (Precision between assays) CV% 5.88%

Recovery for Human APAF-1 ELISA Kit (Colorimetric)

Recovery

84-110%

Linearity

Scientific Data Images for Human APAF-1 ELISA Kit (Colorimetric)

ELISA: Human APAF-1 ELISA Kit (Colorimetric) [NBP2-68232] -

ELISA: Human APAF-1 ELISA Kit (Colorimetric) [NBP2-68232] - Standard Curve Reference

Kit Contents for Human APAF-1 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: APAF-1

Apaf 1 (apoptosis protease-activating factor-1) is a key regulator of the mitochondrial apoptotic pathway (reviewed in Campioni et al, 2005), being the central element of the multimeric apoptosome. The apoptosome consists of cytochrome c, procaspase-9 and seven Apaf 1 monomers, and is considered to be core apoptotic machinery that executes mitochondria-dependent apoptosis. Cytochrome c, normally compartmentalized in the mitochorndria, is released into the cytoplasm following apoptotic stimuli. Apaf 1 binds cytochrome c in the cytoplasm and in the presence of dATP/ATP forms the apoptosome. The apoptosome binds procaspase-9 and promotes its autocatalytic activation. Active caspase-9, in turn, activates downstream caspases including 3, 6, and 7 contributing to the proteolytic caspase activation cascade which leads to cell death. Apaf 1 is an approx. 130 kDa protein. Alternative Apaf 1 splicing isoforms of Apaf 1 have been identified in mammalian cells, each having either or both an 11 amino acid insertion in the N-terminus and an additional WD40 repeat (43 aa) in the C-terminus; please refer to AceView of NCBI (http://www.ncbi.nih.gov/IEB/Research/Acembly/) for detailed information. Additionally, Apaf 1 can be proteolytically processed by active caspase-3 during apoptosis resulting in an Apaf 1 cleavage fragment of ~84 kDa (Lauber et al. 2001).

Long Name

Apoptotic Peptidase Activating Factor 1

Alternate Names

APAF1

Gene Symbol

APAF1

Additional APAF-1 Products

Product Documents for Human APAF-1 ELISA Kit (Colorimetric)

Download Product Datasheets

Download Manual - Human APAF-1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Human APAF-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

Customer Reviews for Human APAF-1 ELISA Kit (Colorimetric)

There are currently no reviews for this product. Be the first to review Human APAF-1 ELISA Kit (Colorimetric) and earn rewards!

Have you used Human APAF-1 ELISA Kit (Colorimetric)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image