APC-PCI Complex Antibody (M36) - BSA Free

Novus Biologicals | Catalog # NBP2-41380

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # M36

Format

BSA Free
View all APC-PCI Complex Primary Antibodies »

Product Specifications

Immunogen

Mixture of PCI in complex with PSA (approx. 80%) and PCI cleaved from such a complex (approx. 20%).

Epitope

The epitope is loctated in the calcium-binding N-terminal EGF domain of protein C.

Specificity

Antibody is specific for APC:PCI complex and cleaved PCI but has little reactivity towards native PCI. The dissociation constants (as determined by standard surface plasmon resonance technique) are: Kd= 4x10e-10 M for PCI in complex with APC and 2x10e-10 M for cleaved PCI. The value of Kd for native PCI is too low to measure, i.e. > 10e-5 M.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for APC-PCI Complex Antibody (M36) - BSA Free

ELISA: APC-PCI Complex Antibody (M36) - BSA Free [NBP2-41380]

ELISA: APC-PCI Complex Antibody (M36) - BSA Free [NBP2-41380]

ELISA: APC-PCI Complex Antibody (M36) - BSA Free [NBP2-41380] - The figure illustrates the calibration curve of a sandwich assay with NBP2-41380 as the capture antibody and biotinylated detection antibody.

Applications for APC-PCI Complex Antibody (M36) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000
Application Notes
ELISA: NBP2-41380 shows excellent reactivity towards PCI in cleaved and complex form in ELISA. NBP2-41380 is a part of the APC-PCI Matched Reagent Set, and can be used in ELISA as a capture antibody in combination with SA001RA as the detection antibody. (1-5) WB: NBP2-41380 shows low reactivity against denatured antigen in SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Product Documents for APC-PCI Complex Antibody (M36) - BSA Free

Certificate of Analysis

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Product Specific Notices for APC-PCI Complex Antibody (M36) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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