Key Product Details

Species Reactivity

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Apc1 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human APC1. The immunogen is located within amino acids 1810 - 1860 of APC1. Amino Acid Squence: SRSHQERLQNHPKRGLF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Apc1 Antibody - BSA Free

Western Blot: Apc1 AntibodyBSA Free [NBP1-77375]

Western Blot: Apc1 AntibodyBSA Free [NBP1-77375]

Western Blot: Apc1 Antibody [NBP1-77375] - analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry-Paraffin: Apc1 Antibody - BSA Free [NBP1-77375]

Immunohistochemistry-Paraffin: Apc1 Antibody - BSA Free [NBP1-77375]

Immunohistochemistry-Paraffin: Apc1 Antibody [NBP1-77375] - Human brain tissue with APC1 antibody at 5 ug/mL.
Flow Cytometry: Apc1 Antibody - BSA Free [NBP1-77375]

Flow Cytometry: Apc1 Antibody - BSA Free [NBP1-77375]

Flow Cytometry: Apc1 Antibody [NBP1-77375] - APC1 expression in human lymphocytes. Image from verified customer review.

Applications for Apc1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2ug/ml
Application Notes
Apc1 antibody validated for FLOW from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP1-77375 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apc1

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC1, also known as mitotic checkpoint regulator (MCPR), is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex.

Alternate Names

anaphase promoting complex subunit 1, anaphase-promoting complex 1 (meiotic checkpoint regulator), anaphase-promoting complex subunit 1, APC1Testis-specific gene 24 protein, Cyclosome subunit 1, Mitotic checkpoint regulator, TSG24MCPR

Gene Symbol

ANAPC1

UniProt

Q9H1A4 (Human)

Additional Apc1 Products

Product Documents for Apc1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Apc1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Apc1 Antibody - BSA Free

Customer Reviews for Apc1 Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human PBMC
    Species: Human
    Verified Customer | Posted 06/30/2014
    APC1 expression in human lymphocytes
    Apc1 Antibody - BSA Free NBP1-77375

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Protocols

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FAQs

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