Apc10 Antibody (8F1D10) - BSA Free

Novus Biologicals | Catalog # NBP2-61889

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8F1D10

Format

BSA Free
View all Apc10 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Apc10 (AA: 1-185) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Apc10 Antibody (8F1D10) - BSA Free

Western Blot: Apc10 Antibody (8F1D10)BSA Free [NBP2-61889]

Western Blot: Apc10 Antibody (8F1D10)BSA Free [NBP2-61889]

Western Blot: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis using ANAPC10 mAb against HEK293 (1) and ANAPC10 (AA: 1-185)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Apc10 Antibody (8F1D10) - BSA Free [NBP2-61889]

Immunocytochemistry/ Immunofluorescence: Apc10 Antibody (8F1D10) - BSA Free [NBP2-61889]

Immunocytochemistry/Immunofluorescence: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis of Hela cells using ANAPC10 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry: Apc10 Antibody (8F1D10) - BSA Free [NBP2-61889]

Immunohistochemistry: Apc10 Antibody (8F1D10) - BSA Free [NBP2-61889]

Immunohistochemistry: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis of paraffin-embedded esophageal cancer tissues using ANAPC10 mouse mAb with DAB staining.
Flow Cytometry: Apc10 Antibody (8F1D10) - BSA Free [NBP2-61889]

Flow Cytometry: Apc10 Antibody (8F1D10) - BSA Free [NBP2-61889]

Flow Cytometry: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis of Hela cells using ANAPC10 mouse mAb (green) and negative control (red).
Western Blot: Apc10 Antibody (8F1D10)BSA Free [NBP2-61889]

Western Blot: Apc10 Antibody (8F1D10)BSA Free [NBP2-61889]

Western Blot: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis using ANAPC10 mAb against human ANAPC10 (AA: 1-185) recombinant protein. (Expected MW is 47.2 kDa)
Western Blot: Apc10 Antibody (8F1D10)BSA Free [NBP2-61889]

Western Blot: Apc10 Antibody (8F1D10)BSA Free [NBP2-61889]

Western Blot: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis using ANAPC10 mouse mAb against Hela (1), MCF-7 (2), SK-Br-3 (3), A431 (4), HEK293 (5), A549 (6), and SPC-A-1 (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: Apc10 Antibody (8F1D10) - BSA Free [NBP2-61889]

Immunocytochemistry/ Immunofluorescence: Apc10 Antibody (8F1D10) - BSA Free [NBP2-61889]

Immunocytochemistry/Immunofluorescence: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis of Hela cells using ANAPC10 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry: Apc10 Antibody (8F1D10) - BSA Free [NBP2-61889]

Immunohistochemistry: Apc10 Antibody (8F1D10) - BSA Free [NBP2-61889]

Immunohistochemistry: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis of paraffin-embedded colon cancer tissues using ANAPC10 mouse mAb with DAB staining.
ELISA: Apc10 Antibody (8F1D10) - BSA Free [NBP2-61889]

ELISA: Apc10 Antibody (8F1D10) - BSA Free [NBP2-61889]

ELISA: Apc10 Antibody (8F1D10) [NBP2-61889] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for Apc10 Antibody (8F1D10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apc10

ANAPC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1; MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]).;

Alternate Names

anaphase promoting complex subunit 10, anaphase-promoting complex subunit 10, APC10DOC1, Cyclosome subunit 10, DKFZP564L0562

Gene Symbol

ANAPC10

Additional Apc10 Products

Product Documents for Apc10 Antibody (8F1D10) - BSA Free

Certificate of Analysis

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Product Specific Notices for Apc10 Antibody (8F1D10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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