Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (91%), Mouse (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 19F461

Format

Azide and BSA Free
View all APIP Primary Antibodies »

Product Specifications

Immunogen

The antibody was developed against a full-length His-tagged recombinant APIP.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (94%), Bovine (91%)

Localization

Cytoplasm, Nucleus

Specificity

This monoclonal antibody can be used for detection of APIP and APIP2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for APIP Antibody (19F461) - Azide and BSA Free

Western Blot: APIP Antibody (19F461)Azide Free [NBP2-33231]

Western Blot: APIP Antibody (19F461)Azide Free [NBP2-33231]

Western Blot: APIP Antibody (19F461) - Azide Free [NBP2-33231] - nalysis of APIP2 in (A) recombinant protein and (B) HeLa cell lysate using APIP antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231] - Analysis of human lung cancer tissue section using APIP antibody (clone 19F461) at a concentration of 5 ug/ml. The representative image shows a nuclear and cytoplasmic staining pattern of APIP expression in lung cancer cells.
Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231] - Formalin-fixed, paraffin-embedded adenocarcinoma of the breast stained with APIP antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231] - Formalin-fixed, paraffin-embedded human stomach stained with APIP antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231] - Analysis of formalin-fixed paraffin-embedded human kidney tissue using APIP antibody at 5 ug/ml concentration.
Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231] - Analysis of APIP in a section of normal prostate from human using 5 ug/ml concentration of APIP antibody (clone 19F461). The tubuloalveolar glands in prostate section depicted APIP positivity in the cytoplasm and nuclei of the epithelial cells. The fibro-muscular stroma showed an overall weak staining with intense nuclear positivity in some cell
Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231] - Detection of APIP in a section of normal human breast tissue using APIP antibody (clone 19F461) at a concentration of 5 ug/ml. The breast's ductal/acinar epithelial cells showed strong cytoplasmic as well as nuclear expression, whereas the myoepithelial cells and the intra-lobular connective tissue depicted very weak to negligible APIP positivity.
Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) - Azide Free [NBP2-33231] - Analysis of human renal cancer tissue section using APIP antibody (clone 19F461) at a concentration of 5 ug/ml. The representative image shows a nuclear and cytoplasmic staining pattern of APIP expression in cancer cells.

Applications for APIP Antibody (19F461) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APIP

The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Recently, Cho et al (4) have identified a new Apaf-1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.

Alternate Names

APAF1 interacting protein, APAF1-interacting protein, APIP2, CGI29, CGI-29, dJ179L10.2, EC 4.2.1.109, MMRP19, MTRu-1-P dehydratase, probable methylthioribulose-1-phosphate dehydratase

Gene Symbol

APIP

Additional APIP Products

Product Documents for APIP Antibody (19F461) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for APIP Antibody (19F461) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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