Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 19F461
View all APIP Primary Antibodies »

Product Specifications

Immunogen

The antibody was developed against a full-length His-tagged recombinant APIP.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (91%) Use in Mouse reported in scientific literature (PMID:31263105).

Localization

Cytoplasm, Nucleus

Specificity

This monoclonal antibody can be used for detection of APIP and APIP2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for APIP Antibody (19F461)

Western Blot: APIP Antibody (19F461) [NB100-56567]

Western Blot: APIP Antibody (19F461) [NB100-56567]

Western Blot: APIP Antibody (19F461) [NB100-56567] - Analysis of APIP2 in (A) recombinant protein and (B) HeLa cell lysate using APIP antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Analysis of human lung cancer tissue section using APIP antibody (clone 19F461) at a concentration of 5 ug/ml. The representative image shows a nuclear and cytoplasmic staining pattern of APIP expression in lung cancer cells.
Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Formalin-fixed, paraffin-embedded adenocarcinoma of the breast stained with APIP antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Formalin-fixed, paraffin-embedded human stomach stained with APIP antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Analysis of formalin-fixed paraffin-embedded human kidney tissue using APIP antibody at 5 ug/ml concentration.
Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Analysis of APIP in a section of normal prostate from human using 5 ug/ml concentration of APIP antibody (clone 19F461). The tubuloalveolar glands in prostate section depicted APIP positivity in the cytoplasm and nuclei of the epithelial cells. The fibro-muscular stroma showed an overall weak staining with intense nuclear positivity in some cells.
Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Detection of APIP in a section of normal human breast tissue using APIP antibody (clone 19F461) at a concentration of 5 ug/ml. The breast's ductal/acinar epithelial cells showed strong cytoplasmic as well as nuclear expression, whereas the myoepithelial cells and the intra-lobular connective tissue depicted very weak to negligible APIP positivity.
Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Analysis of human renal cancer tissue section using APIP antibody (clone 19F461) at a concentration of 5 ug/ml. The representative image shows a nuclear and cytoplasmic staining pattern of APIP expression in cancer cells.

Applications for APIP Antibody (19F461)

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APIP

The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis3. Recently, Cho et al have identified a new Apaf-1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.

Alternate Names

APAF1 interacting protein, APAF1-interacting protein, APIP2, CGI29, CGI-29, dJ179L10.2, EC 4.2.1.109, MMRP19, MTRu-1-P dehydratase, probable methylthioribulose-1-phosphate dehydratase

Entrez Gene IDs

51074 (Human); 56369 (Mouse); 508345 (Bovine)

Gene Symbol

APIP

UniProt

Q96GX9 (Human)

Additional APIP Products

Product Documents for APIP Antibody (19F461)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for APIP Antibody (19F461)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for APIP Antibody (19F461)

Customer Reviews for APIP Antibody (19F461)

There are currently no reviews for this product. Be the first to review APIP Antibody (19F461) and earn rewards!

Have you used APIP Antibody (19F461)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies