Q: I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?

A: All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.

Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-68246

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Product Documents

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

2.81 ng/mL

Assay Range

4.69 - 300 ng/mL

View all Apolipoprotein A-IV/ApoA4 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human ApoA4 in samples. No significant cross-reactivity or interference between Human ApoA4 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.09%

Inter-Assay Precision (Precision between assays) CV% 5.5%

Recovery for Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

Recovery

88-103%

Linearity

Scientific Data Images for Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

ELISA: Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric) [NBP2-68246] -

ELISA: Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric) [NBP2-68246] - Standard Curve Reference

Kit Contents for Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Apolipoprotein A-IV/ApoA4

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Alternate Names

Apolipoprotein AIV

Gene Symbol

APOA4

Additional Apolipoprotein A-IV/ApoA4 Products

Product Documents for Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

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Download Manual - Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Obesity

Citations for Human Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

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