Rat Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-68247

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat ApoA4 in samples. No significant cross-reactivity or interference between Rat ApoA4 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.77%

Inter-Assay Precision (Precision between assays) CV% 5.46%

Recovery for Rat Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

Recovery

87-107%

Linearity

Rat Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

Rat Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

ELISA: Rat Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric) [NBP2-68247] -

ELISA: Rat Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric) [NBP2-68247] - Standard Curve Reference

Kit Contents for Rat Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Apolipoprotein A-IV/ApoA4

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Alternate Names

Apolipoprotein AIV

Gene Symbol

APOA4

Additional Apolipoprotein A-IV/ApoA4 Products

Product Documents for Rat Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

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FAQs for Rat Apolipoprotein A-IV/ApoA4 ELISA Kit (Colorimetric)

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  • Q: I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?

    A:

    All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.

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