Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

Key Product Details

Product Specifications

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.60%

Inter-Assay Precision (Precision between assays) CV% 7.60%

Recovery for Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

Scientific Data Images for Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

Kit Contents for Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

• Biotinylated Human Apolipoprotein E Antibody (50x): A 50-fold concentrated biotinylated polyclonal antibody against human Apo-E.
• Chromogen Substrate (1x): A stabilized peroxidase chromogen substrate tetramethylbenzidine.
• Human Apolipoprotein E Microplate: A 96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against human Apo-E.
• Human Apolipoprotein E Standard: Human Apo-E in a buffered protein base, lyophilized.
• MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
• Sealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay.
• SP Conjugate (100x): A 100-fold concentrate.
• Standard Diluent (1x): A buffered protein base with stabilizer.
• Stop Solution (1x): A 0.5 N hydrochloric acid solution to stop the chromogen substrate reaction.
• Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Preparation and Storage

Background: Apolipoprotein E/ApoE

Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.

The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.

Additional Apolipoprotein E/ApoE Products

• All Products for Apolipoprotein E/ApoE
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• Apolipoprotein E/ApoE Primary Antibodies
• Apolipoprotein E/ApoE Proteins and Enzymes

Product Documents for Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric)