Human APP ELISA Kit (Chemiluminescence)

Novus Biologicals | Catalog # NBP2-66740

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Saliva, Urine, Milk, Cell Supernatant, Tissue Homogenate (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63-1000 pg/mL
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Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Species

Human

Specificity

This kit recognizes Human APP in samples. No significant cross-reactivity or interference between Human APP and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 10%

Inter-Assay Precision (Precision between assays) CV% 9.48%

Recovery for Human APP ELISA Kit (Chemiluminescence)

Recovery

84-103%

Linearity

Human APP ELISA Kit (Chemiluminescence)

Scientific Data Images for Human APP ELISA Kit (Chemiluminescence)

Human APP ELISA Kit (Chemiluminescence)

ELISA: Human APP ELISA Kit (Chemiluminescence) [NBP2-66740] -

ELISA: Human APP ELISA Kit (Chemiluminescence) [NBP2-66740] - Standard Curve Reference

Kit Contents for Human APP ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: APP

Alzheimer's disease (AD) is characterized by the presence of senile plaques in the brain and blood vessel walls. Amyloid Beta A4 (A 4) protein has been found to be the principal constituent of senile plaques of AD's patients (1-2). The amyloid beta-protein precursor (APP) is proteolytically cleaved to generate the 4 kDa A 4 protein. Two variants of A 4 can be detected; A 1-40 "short-tailed" and A 1-42 "long-tailed" (3-4).

Long Name

Amyloid Precursor Protein

Alternate Names

Amyloid beta, beta Amyloid, Protease Nexin II

Gene Symbol

APP

Additional APP Products

Product Documents for Human APP ELISA Kit (Chemiluminescence)

Certificate of Analysis

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Product Specific Notices for Human APP ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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