Rat APP ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-78747

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat APP in samples. No significant cross-reactivity or interference between Rat APP and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.33%

Inter-Assay Precision (Precision between assays) CV% 4.82%

Recovery for Rat APP ELISA Kit (Colorimetric)

Recovery

89-105%

Linearity

Rat APP ELISA Kit (Colorimetric)

Scientific Data Images for Rat APP ELISA Kit (Colorimetric)

Rat APP ELISA Kit (Colorimetric)

ELISA: Rat APP ELISA Kit (Colorimetric) [NBP2-78747] -

ELISA: Rat APP ELISA Kit (Colorimetric) [NBP2-78747] - Standard Curve Reference

Kit Contents for Rat APP ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: APP

Alzheimer's disease (AD) is characterized by the presence of senile plaques in the brain and blood vessel walls. Amyloid Beta A4 (A 4) protein has been found to be the principal constituent of senile plaques of AD's patients (1-2). The amyloid beta-protein precursor (APP) is proteolytically cleaved to generate the 4 kDa A 4 protein. Two variants of A 4 can be detected; A 1-40 "short-tailed" and A 1-42 "long-tailed" (3-4).

Long Name

Amyloid Precursor Protein

Alternate Names

Amyloid beta, beta Amyloid, Protease Nexin II

Gene Symbol

APP

UniProt

P08592 (Rat)

Additional APP Products

Product Documents for Rat APP ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat APP ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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