ARFGAP3 Antibody

Novus Biologicals | Catalog # NB100-843

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Bovine (100%), Canine (100%), Human (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ARFGAP3 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NGVVTSIQDRYGS corresponding to C-Terminus according to NP_055385.3, NP_001135765.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_055385.3; NP_001135765.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ARFGAP3 Antibody

Western Blot: ARFGAP3 Antibody [NB100-843]

Western Blot: ARFGAP3 Antibody [NB100-843]

Western Blot: ARFGAP3 Antibody [NB100-843] - (2ug/ml) of mouse brain extracts (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for ARFGAP3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 55-60 kDa band observed in mouse brain extracts.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ARFGAP3

The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which is thought to interact with ADP-ribosylation factor 1 (ARF1). The encoded protein likely promotes hydrolysis of ARF1-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene.

Alternate Names

ADP-ribosylation factor GTPase activating protein 1, ADP-ribosylation factor GTPase activating protein 3, ADP-ribosylation factor GTPase-activating protein 3, ARF GAP 3, ARFGAP1, FLJ45618

Entrez Gene IDs

55738 (Human); 66251 (Mouse)

Gene Symbol

ARFGAP1

UniProt

NP_055385. (Human)

Additional ARFGAP3 Products

Product Documents for ARFGAP3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ARFGAP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ARFGAP3 Antibody

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