Aromatase Antibody

Novus Biologicals | Catalog # NB100-93375

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Aromatase Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HDLSLHPDETKN corresponding to internal region according to NP_000094.2, NP_112503.1.

Epitope

C-HDLSLHPDETKN

Specificity

This antibody is expected to recognise both reported isoforms (NP_000094.2 and NP_112503.1), which are identical

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Aromatase Antibody

Western Blot: Aromatase Antibody [NB100-93375]

Western Blot: Aromatase Antibody [NB100-93375]

Western Blot: Aromatase Antibody [NB100-93375] - Analysis of Aromatase using this antibody at 0.1 ug/ml in Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Aromatase Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 50-55 kDa band observed in human placenta lysates (calculated MW of 57.9 kDa band according to NP_000094.2 and NP_112503.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aromatase

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis, three successive hydroxylations of the A ring of androgens. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. The gene expresses two transcript variants. [provided by RefSeq]

Long Name

Aromatase

Alternate Names

ARO1, CYAR, CYP19, CYP19A1, CYPXIX, EC 1.14.14.14, Estrogen synthase

Entrez Gene IDs

1588 (Human)

Gene Symbol

CYP19A1

UniProt

NP_000094.2 (Human)

Additional Aromatase Products

Product Documents for Aromatase Antibody

Certificate of Analysis

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Product Specific Notices for Aromatase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Aromatase Antibody

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