![Western Blot: Atad5 Antibody [NB100-57495]](https://resources.rndsystems.com/images/products/Atad5-Antibody-Western-Blot-NB100-57495-img0001.jpg "Western Blot: Atad5 Antibody [NB100-57495]")
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 775 and 825 of human ATPase family, AAA domain containing 5 using the numbering given in entry NP_079133.3 (GeneID 79915).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Atad5 Antibody
Western Blot: Atad5 Antibody [NB100-57495]
Western Blot: Atad5 Antibody [NB100-57495] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLaand HEK293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-ATAD5/FRAG1 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).Applications for Atad5 Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Atad5
Long Name
ATPase family AAA domain-containing protein 5
Alternate Names
C17orf41, FRAG1
Entrez Gene IDs
79915 (Human)
Gene Symbol
ATAD5
UniProt
Additional Atad5 Products
Product Documents for Atad5 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Atad5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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