ATF1 [p Ser63] Antibody

Novus Biologicals | Catalog # NBP3-12920

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all ATF1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser63 of human ATF1. The exact sequence is proprietary.

Modification

p Ser 63

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for ATF1 [p Ser63] Antibody

Western Blot: ATF1 [p Ser63] Antibody [NBP3-12920]

Western Blot: ATF1 [p Ser63] Antibody [NBP3-12920]

Western Blot: ATF1 [p Ser63] Antibody [NBP3-12920] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ATF1 (phospho Ser63) antibody (NBP3-12920) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: ATF1 [p Ser63] Antibody [NBP3-12920]

Western Blot: ATF1 [p Ser63] Antibody [NBP3-12920]

Western Blot: ATF1 [p Ser63] Antibody [NBP3-12920] - Untreated (-) and treated (+) 293T whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF1 (phospho Ser63) antibody (NBP3-12920) diluted at 1:5000.
Western Blot: ATF1 [p Ser63] Antibody [NBP3-12920]

Western Blot: ATF1 [p Ser63] Antibody [NBP3-12920]

Western Blot: ATF1 [p Ser63] Antibody [NBP3-12920] - Untreated (-) and treated (+) A431 whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF1 (phospho Ser63) antibody (NBP3-12920) diluted at 1:5000.

Applications for ATF1 [p Ser63] Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATF1

ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.

Long Name

Activating Transcription Factor 1

Alternate Names

TREB36

Gene Symbol

ATF1

Additional ATF1 Products

Product Documents for ATF1 [p Ser63] Antibody

Certificate of Analysis

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Product Specific Notices for ATF1 [p Ser63] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail