ATF4 Antibody

Novus Biologicals | Catalog # NB100-852

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ATF4 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EEVRKARGKKRVP corresponding to C-Terminus according to NP_877962.1.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Porcine.

Specificity

This antibody is expected to recognize both reported isoforms (NP_001666.2; NP_877962.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

38.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATF4 Antibody

Western Blot: ATF4 Antibody [NB100-852]

Western Blot: ATF4 Antibody [NB100-852]

Western Blot: ATF4 Antibody [NB100-852] - Staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Western Blot: ATF4 Antibody [NB100-852]

Western Blot: ATF4 Antibody [NB100-852]

Western Blot: ATF4 Antibody [NB100-852] - Ethanol feeding induced ATF4 expression in mouse liver which was suppressed by Lipin1 overexpression in adipose tissue. Western blot image submitted by a verified customer review.

Applications for ATF4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1 ug/mL
Application Notes
Western blot: Approx 35-37 kDa band observed in Mouse Heart lysates (calculated MW of 38.3 kDa according to Mouse NP_001274109.1; NP_033846.2). A customer has reported positive results on mouse embryonic fibroblasts (3T3 cells).

Reviewed Applications

Read 1 review rated 5 using NB100-852 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATF4

ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain (referenced from Entrez gene).

Long Name

Activating Transcription Factor 4

Alternate Names

TAXREB67, TXREB

Entrez Gene IDs

468 (Human); 11911 (Mouse); 79255 (Rat)

Gene Symbol

ATF4

UniProt

P18848 (Human)

Additional ATF4 Products

Product Documents for ATF4 Antibody

Certificate of Analysis

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Product Specific Notices for ATF4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ATF4 Antibody (1)

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1 Customer Rating
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Customer Images

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  • ATF4 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse whole liver
    Species: Mouse
    Verified Customer | Posted 02/06/2018
    ethanol feeding induced ATF4 expression in mouse liver which was suppressed by Lipin1 overexpression in adipose tissue.
    ATF4 Antibody NB100-852

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways
TGF-beta Signaling Pathway Thumbnail