ATF5 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92397

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all ATF5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 101-282 of human ATF5 (NP_036200.2). MASLLKKELEQMEDFFLDAPPLPPPSPPPLPPPPLPPAPSLPLSLPSFDLPQPPVLDTLDLLAIYCRNEAGQEEVGMPPLPPPQQPPPPSPPQPSRLAPYPHPATTRGDRKQKKRDQNKSAALRYRQRKRAEGEALEGECQGLEARNRELKERAESVEREIQYVKDLLIEVYKARSQRTRSC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATF5 Antibody - Azide and BSA Free

Western Blot: ATF5 AntibodyAzide and BSA Free [NBP2-92397]

Western Blot: ATF5 AntibodyAzide and BSA Free [NBP2-92397]

Western Blot: ATF5 Antibody [NBP2-92397] - Analysis of extracts of rat lung, using ATF5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
Immunohistochemistry-Paraffin: ATF5 Antibody - Azide and BSA Free [NBP2-92397]

Immunohistochemistry-Paraffin: ATF5 Antibody - Azide and BSA Free [NBP2-92397]

Immunohistochemistry-Paraffin: ATF5 Antibody [NBP2-92397] - Rat ovary using ATF5 Rabbit pAb (NBP2-92397) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: ATF5 Antibody - Azide and BSA Free [NBP2-92397]

Immunohistochemistry-Paraffin: ATF5 Antibody - Azide and BSA Free [NBP2-92397]

Immunohistochemistry-Paraffin: ATF5 Antibody [NBP2-92397] - Mouse lung using ATF5 Rabbit pAb (NBP2-92397) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ATF5 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: ATF5 Antibody - Azide and BSA Free [NBP2-92397] -

Immunocytochemistry/ Immunofluorescence: ATF5 Antibody - Azide and BSA Free [NBP2-92397] - Immunofluorescence analysis of U2OS cells using ATF5 Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
ATF5 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: ATF5 Antibody - Azide and BSA Free [NBP2-92397] -

Immunocytochemistry/ Immunofluorescence: ATF5 Antibody - Azide and BSA Free [NBP2-92397] - Immunofluorescence analysis of PC-12 cells using ATF5 Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for ATF5 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATF5

Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4

Alternate Names

activating transcription factor 5FLJ34666, ATFXHMFN0395, cAMP-dependent transcription factor ATF-5, cyclic AMP-dependent transcription factor ATF-5, Transcription factor ATFx

Gene Symbol

ATF5

Additional ATF5 Products

Product Documents for ATF5 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ATF5 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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