![Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501]](https://resources.rndsystems.com/images/products/ATF6-Antibody-Immunohistochemistry-Paraffin-NBP2-48501-img0008.jpg "Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (91%), Rat (94%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: EAQDTSDGIIQKNSYRYDHSVSNDKALMVLTEEPLLYIPPPPCQPLINTTESLRLNHELRGWVHRHEVERTKSRRMTNNQQKTRILQGALEQGSNSQLMAVQYTETTSSISRNSGSELQVYYASP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ATF6 Antibody - BSA Free
Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501]
Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501] - Staining of human testis shows moderate cytoplasmic positivity in a subset of cells in seminiferous ducts.![Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501]](https://resources.rndsystems.com/images/products/ATF6-Antibody-Immunohistochemistry-Paraffin-NBP2-48501-img0005.jpg "Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501]")
Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501]
Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.![Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501]](https://resources.rndsystems.com/images/products/ATF6-Antibody-Immunohistochemistry-Paraffin-NBP2-48501-img0006.jpg "Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501]")
Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501]
Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.![Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501]](https://resources.rndsystems.com/images/products/ATF6-Antibody-Immunohistochemistry-Paraffin-NBP2-48501-img0007.jpg "Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501]")
Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501]
Immunohistochemistry-Paraffin: ATF6 Antibody [NBP2-48501] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Chromatin Immunoprecipitation ChIP: ATF6 Antibody - BSA Free
ChIP-Exo-Seq composite graph for Anti-ATF6 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.Applications for ATF6 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ATF6
Long Name
Activating Transcription Factor 6
Alternate Names
ACHM7, ATF6 alpha
Gene Symbol
ATF6
Additional ATF6 Products
Product Documents for ATF6 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ATF6 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for ATF6 Antibody - BSA Free
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Q: We are looking for an antibody specific to ATF6 alpha that will not cross-react with ATF6 beta. Would you please help confirm if any of the following are ATF6 alpha specific antibodies: NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439?
A: The antibodies NBP1-77251 and NBP1-76675 are specific to ATF6 alpha and should not cross-react with ATF6 beta.
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