ATG10 Antibody (JE59-86)

Novus Biologicals | Catalog # NBP3-32055

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE59-86 expressed in HEK293
View all ATG10 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to Human ATG10 aa 180-220 (C terminal) (Uniprot: Q9H0Y0)

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATG10 Antibody (JE59-86)

ATG10 Antibody (JE59-86)

Western Blot: ATG10 Antibody (JE59-86) [NBP3-32055]

Western Blot: ATG10 Antibody (JE59-86) [NBP3-32055] - Western blot analysis of ATG10 on different lysates with Rabbit anti-ATG10 antibody (NBP3-32055) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: K-562 cell lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 25 kDa
Observed band size: 30 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32055) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for ATG10 Antibody (JE59-86)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATG10

Atg10 is a major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. APG10 interacts with LC3A to facilitate the conjugation of ATG12 to ATG5. APG10 is also able to directly interact either with ATG5 or ATG7A. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies.

Long Name

Autophagy Related 10 Homolog

Alternate Names

APG10, APG10L, PP12616

Gene Symbol

ATG10

Additional ATG10 Products

Product Documents for ATG10 Antibody (JE59-86)

Certificate of Analysis

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Product Specific Notices for ATG10 Antibody (JE59-86)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy

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