Recombinant Human ATG10 His Protein

Novus Biologicals | Catalog # NBP2-22702

Novus Biologicals
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Key Product Details

Source

E. coli

Accession Number

Q9H0Y0

Tag

His

Applications

SDS-PAGE
View all ATG10 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-220 of Human ATG10

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEEDEFI GEKTFQRYCA EFIKHSQQIG DSWEWRPSKD CSDGYMCKIH FQIKNGSVMS HLGASTHGQT CLPMEEAFEL PLDDCEVIET AAASEVIKYE YHVLYSCSYQ VPVLYFRASF LDGRPLTLKD IWEGVHECYK MRLLQGPWDT ITQQEHPILG QPFFVLHPCK TNEFMTPVLK NSQKINKNVN YITSWLSIVG PVVGLNLPLS YAKATSQDER NVP

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

27.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human ATG10 His Protein

SDS-PAGE: Recombinant Human ATG10 His Protein [NBP2-22702]

SDS-PAGE: Recombinant Human ATG10 His Protein [NBP2-22702]

SDS-Page: Atg10 Protein [NBP2-22702]

Formulation, Preparation, and Storage

NBP2-22702
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 10% glycerol, 1 mM DTT
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATG10

Ubiquitin-like-conjugating enzyme ATG10, also known as ATG10, is a 220 amino acid protein that localizes to the cytoplasm and plays a role in autophagy, specifically functioning as an E2-like enzyme that provides Atg recognition sites during autophagosome synthesis. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. Recombinant human ATG10 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Autophagy Related 10 Homolog

Alternate Names

APG10, APG10L, PP12616

Gene Symbol

ATG10

UniProt

Q9H0Y0

Additional ATG10 Products

Product Documents for Recombinant Human ATG10 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human ATG10 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy

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