ATP6AP1 Antibody (5G12A4) - BSA Free

Novus Biologicals | Catalog # NBP3-27174

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5G12A4

Format

BSA Free
View all ATP6AP1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human ATP6AP1 (AA: 51-151) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATP6AP1 Antibody (5G12A4) - BSA Free

Western Blot: ATP6AP1 Antibody (5G12A4) [NBP3-27174] -

Western Blot: ATP6AP1 Antibody (5G12A4) [NBP3-27174] - Analysis using ATP6AP1 mAb against human ATP6AP1 (AA: 51-151) recombinant protein. (Expected MW is 37kDa)

ELISA: ATP6AP1 Antibody (5G12A4) [NBP3-27174] -

ELISA: ATP6AP1 Antibody (5G12A4) [NBP3-27174] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Western Blot: ATP6AP1 Antibody (5G12A4) [NBP3-27174] -

Western Blot: ATP6AP1 Antibody (5G12A4) [NBP3-27174] - Analysis using ATP6AP1 mAb against HEK293-6e (1) and human ATP6AP1 (AA: 51-151)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western Blot: ATP6AP1 Antibody (5G12A4) [NBP3-27174] -

Western Blot: ATP6AP1 Antibody (5G12A4) [NBP3-27174] - Analysis using ATP6AP1 mouse mAb against PC-3 (1) cell lysate.

Flow Cytometry: ATP6AP1 Antibody (5G12A4) [NBP3-27174] -

Flow Cytometry: ATP6AP1 Antibody (5G12A4) [NBP3-27174] - Analysis of Hela cells using ATP6AP1 mouse mAb (green) and negative control (red).

Applications for ATP6AP1 Antibody (5G12A4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP6AP1

ATP6AP1 encodes a component of a multisubunit enzyme (1 mDa MW) that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene is approximately 45 kD and may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules.

Alternate Names

16A, Ac45, ATP6IP1V-ATPase subunit S1, ATP6S1ORF, ATPase, H+ transporting, lysosomal (vacuolar proton pump), subunit 1, ATPase, H+ transporting, lysosomal accessory protein 1, H+ transporting, lysosomal interacting protein 1, H-ATPase subunit, MGC129781, Protein XAP-3, Vacuolar proton pump subunit S1, VATPS1V-ATPase Ac45 subunit, V-type proton ATPase subunit S1, XAP-3, XAP3V-ATPase S1 accessory protein

Gene Symbol

ATP6AP1

Additional ATP6AP1 Products

Product Documents for ATP6AP1 Antibody (5G12A4) - BSA Free

Certificate of Analysis

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Product Specific Notices for ATP6AP1 Antibody (5G12A4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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