ATRX Antibody (CL0537) - BSA Free

Novus Biologicals | Catalog # NBP2-52938

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (96%), Rat (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0537

Format

BSA Free
View all ATRX Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P46100, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ATRX Antibody (CL0537) - BSA Free

Western Blot: ATRX Antibody (CL0537) [NBP2-52938]

Western Blot: ATRX Antibody (CL0537) [NBP2-52938]

Western Blot: ATRX Antibody (CL0537) [NBP2-52938] - analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATRX antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938]

Immunocytochemistry/ Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938]

Immunocytochemistry/Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938] - Staining in U251 cell line with Anti-ATRX monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938]

Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938]

Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunocytochemistry/ Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938]

Immunocytochemistry/ Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938]

Immunocytochemistry/Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938] - Staining in A431 cell line with Anti-ATRX monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunocytochemistry/ Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938]

Immunocytochemistry/ Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938]

Immunocytochemistry/Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938] - Staining in HeLa cell line with Anti-ATRX monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunocytochemistry/ Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938]

Immunocytochemistry/ Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938]

Immunocytochemistry/Immunofluorescence: ATRX Antibody (CL0537) [NBP2-52938] - Staining in MCF7 cell line with Anti-ATRX monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938]

Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938]

Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938]

Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938]

Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938] - Staining of human glioma shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938]

Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938]

Immunohistochemistry-Paraffin: ATRX Antibody (CL0537) [NBP2-52938] - Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts and Leydig cells.

Applications for ATRX Antibody (CL0537) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATRX

ATRX is encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. The mutations of this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.

Alternate Names

alpha thalassemia/mental retardation syndrome X-linked, alpha thalassemia/mental retardation syndrome X-linked (RAD54 (S. cerevisiae)homolog), alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S.cerevisiae), ATP-dependent helicase ATRX, ATR2, DNA dependent ATPase and helicase, EC 3.6.1, EC 3.6.4.12, helicase 2, X-linked, Juberg-Marsidi syndrome, MGC2094, MRXHF1, RAD54 homolog, RAD54L, SFM1, SHS, transcriptional regulator ATRX, XH2RAD54, X-linked helicase II, X-linked nuclear protein, XNPZNF-HX, Zinc finger helicase, Znf-HX

Gene Symbol

ATRX

Additional ATRX Products

Product Documents for ATRX Antibody (CL0537) - BSA Free

Certificate of Analysis

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Product Specific Notices for ATRX Antibody (CL0537) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ATRX Antibody (CL0537) - BSA Free

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Protocols

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