B220/CD45R Antibody (GS5A)

Name: B220/CD45R Antibody (GS5A)
Brand: Novus Biologicals
SKU: NBP2-60794
Price: 299 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-60794

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Alpaca, Bison, Bovine, Goat, Sheep

Cited:

Bovine

Applications

Validated:

Flow Cytometry

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # GS5A

View all B220/CD45R Primary Antibodies »

Product Specifications

Immunogen

Goat and sheep mononuclear leukocytes

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

This monoclonal antibody is made in ascites form using mice. The ascites is collected and centrifuged to remove cells and large particles. The ascitic fluid is purified further by filtration through sequential filters with the last filter at 2 um. The concentration of the monoclonal antibody present in the ascites is determined with commercial radial immunodiffusion kits (1mg/ml).

Scientific Data Images for B220/CD45R Antibody (GS5A)

Flow Cytometry: B220/CD45R Antibody (GS5A) [NBP2-60794]

Flow Cytometry: B220/CD45R Antibody (GS5A) [NBP2-60794] - Profile obtained with bovine leukocytes.

Applications for B220/CD45R Antibody (GS5A)

Application

Recommended Usage

Flow Cytometry

1:10 - 1:1000

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Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B220/CD45R

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognizing a common epitope on all of the isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.

Long Name

Cluster of Differentiation 45

Alternate Names

CD45R

Gene Symbol

PTPRC

Additional B220/CD45R Products

Product Documents for B220/CD45R Antibody (GS5A)

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Certificate of Analysis

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Product Specific Notices for B220/CD45R Antibody (GS5A)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.