B7-H4 Antibody (188) - BSA Free

Novus Biologicals | Catalog # NBP1-43266

Novus Biologicals

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2b Kappa Clone # 188

Format

BSA Free
View all B7-H4 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was Purified B7H4.

Reactivity Notes

This antibody is reactive to Mouse.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Description

Novus Biologicals Rat B7-H4 Antibody (188) - BSA Free (NBP1-43266) is a monoclonal antibody validated for use in Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for B7-H4 Antibody (188) - BSA Free

Flow Cytometry: B7-H4 Antibody (188) [NBP1-43266]

Flow Cytometry: B7-H4 Antibody (188) [NBP1-43266]

Flow Cytometry: B7-H4 Antibody (188) [NBP1-43266] - Staining of non-transfected (left) and mouse B7-H4 transfected (right) L5178Y cells with 0.25 ug Rat IgG2b kappa Isotype Control Purified (open histogram) or 0.25 ug Anti-Mouse B7-H4 Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.

Applications for B7-H4 Antibody (188) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000
Application Notes
The 188 antibody has been tested by flow cytometric analysis of transfected cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: B7-H4

The 188 monoclonal antibody was generated against and reacts with mouse B7-H4 also known as B7S1, B7X. Cross reactivity of this antibody to other proteins has not been determined. B7-H4 is a newly discovered member of the B7 family reported to inhibit T cell activation, cell cycle progression and IL-2 production. The ligand for B7-H4 has not been identified yet. Simultaneous double staining of cells with two anti-mouse B7-H4 antibodies, and, suggests that epitopes recognized by these mAbs are different and/or there is no steric hindrance when antibodies are used together. 188 stains mouse B7-H4 transfected cells and not spleen cells. Exact expression pattern of B7-H4 has not been fully characterized.

Long Name

B7 Homolog 4

Alternate Names

B7H4, B7S1, B7x, Vtcn1

Gene Symbol

VTCN1

Additional B7-H4 Products

Product Documents for B7-H4 Antibody (188) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for B7-H4 Antibody (188) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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