BAAT Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92503

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BAAT Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 309-418 of human BAAT (NP_001692.1). YLFPIEEAQGQFLFIVGEGDKTINSKAHAEQAIGQLKRHGKNNWTLLSYPGAGHLIEPPYSPLCCASTTHDLRLHWGGEVIPHAAAQEHAWKEIQRFLRKHLIPDVTSQL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BAAT Antibody - BSA Free

Western Blot: BAAT AntibodyBSA Free [NBP2-92503]

Western Blot: BAAT AntibodyBSA Free [NBP2-92503]

Western Blot: BAAT Antibody [NBP2-92503] - Analysis of extracts of various cell lines, using BAAT at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: BAAT Antibody - BSA Free [NBP2-92503]

Immunocytochemistry/ Immunofluorescence: BAAT Antibody - BSA Free [NBP2-92503]

Immunocytochemistry/Immunofluorescence: BAAT Antibody [NBP2-92503] - Analysis of Hep G2 cells using BAAT Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: BAAT Antibody - BSA Free [NBP2-92503]

Immunohistochemistry-Paraffin: BAAT Antibody - BSA Free [NBP2-92503]

Immunohistochemistry-Paraffin: BAAT Antibody [NBP2-92503] - Human liver using BAAT Rabbit pAb at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for BAAT Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BAAT

BAAT is encoded by this gene is a liver enzyme that catalyzes the transfer of C24 bile acids from the acyl-CoA thioester to either glycine or taurine, the second step in the formation of bile acid-amino acid conjugates. The bile acid conjugates then act as a detergent in the gastrointestinal tract, which enhances lipid and fat-soluble vitamin absorption. Defects in this gene are a cause of familial hypercholanemia (FHCA). Two transcript variants encoding the same protein have been found for this gene.

Alternate Names

BACAT, BATbile acid-CoA:amino acid N-acyltransferase, bile acid CoA: amino acid N-acyltransferase (glycine N-choloyltransferase), bile acid Coenzyme A: amino acid N-acyltransferase (glycineN-choloyltransferase), EC 2.3.1.65, EC 3.1.2.2, FLJ20300, Glycine N-choloyltransferase, Long-chain fatty-acyl-CoA hydrolase, MGC104432

Gene Symbol

BAAT

Additional BAAT Products

Product Documents for BAAT Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BAAT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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