BAF57 Antibody

Novus Biologicals | Catalog # NB100-1297

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all BAF57 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PPTDPIPEDEKKE corresponding to C-Terminus according to NP_003070.3.

Localization

Nuclear

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for BAF57 Antibody

Western Blot: BAF57 Antibody [NB100-1297]

Western Blot: BAF57 Antibody [NB100-1297]

Western Blot: BAF57 Antibody [NB100-1297] - Western blot analysis of BAF57 using NB100-1297 in Jurkat lysate (RIPA buffer, 35ug total protein per lane) at 0.2ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: BAF57 Antibody [NB100-1297]

Immunohistochemistry-Paraffin: BAF57 Antibody [NB100-1297]

Immunohistochemistry-Paraffin: BAF57 Antibody [NB100-1297] - In paraffin embedded Human Prostate shows nuclear staining in secretory epithelial cells.
Western Blot: BAF57 Antibody [NB100-1297]

Western Blot: BAF57 Antibody [NB100-1297]

Western Blot: BAF57 Antibody [NB100-1297] - A) HEK293 overexpressing BAF57 and probed with (mock transfection in first lane). B) see Western Blot.

Applications for BAF57 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 5 ug/ml

Immunohistochemistry-Paraffin

3 - 5 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.2 - 0.6 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in lysates of HEPG2, HeLa and Jurkat (calculated MW of 46.6 kDa band according to NP_003070.3). A 48 kDa band was observed in transfected HEK293 transiently expressing BAF57. This band is not observed in the non-transfected HEK293. IHC-P: Human prostate shows nuclear staining in secretory epithelial cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BAF57

The SWI/SNF complex in S. cerevisiae and Drosophila is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. The complex contains an ATP-dependent nucleosome disruption activity that can lead to enhanced binding of transcription factors. The BRG1/brm associated factors, or BAF, complex in mammals is functionally related to SWI/SNF and consists of 9 to 12 subunits, some of which are homologous to SWI/SNF. A 57kD BAF subunit, BAF57, is present in higher eukaryotes, but not in yeast.

Alternate Names

BAF57BRG1-associated factor 57, chromatin remodeling complex BRG1-associated factor 57, subfamily e, member 1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily E member 1

Entrez Gene IDs

6605 (Human); 57376 (Mouse)

Gene Symbol

SMARCE1

UniProt

NP_003070. (Human)

Additional BAF57 Products

Product Documents for BAF57 Antibody

Certificate of Analysis

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Product Specific Notices for BAF57 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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