BAF57 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-90012
![Immunocytochemistry/ Immunofluorescence: BAF57 Antibody [NBP1-90012]](https://resources.rndsystems.com/images/products/BAF57-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90012-img0009.jpg "Immunocytochemistry/ Immunofluorescence: BAF57 Antibody [NBP1-90012]")
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EEREKEAAEQAERSQSSIVPEEEQAANKGEEKKDDENIPMETEETHLEETTESQQNGEEGTSTPEDKESGQEGVDSMAEEGTSDSNTGSESNSATVEEPPTDPIPEDEK
Reactivity Notes
Reactivity reported in scientific literature (PMID: 23435261)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for BAF57 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: BAF57 Antibody [NBP1-90012]
Immunocytochemistry/Immunofluorescence: BAF57 Antibody [NBP1-90012] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.![BAF57 Antibody - BSA Free Immunohistochemistry-Paraffin: BAF57 Antibody - BSA Free [NBP1-90012]](https://resources.rndsystems.com/images/products/nbp1-90012_rabbit-polyclonal-baf57-antibody-15520258544750.jpg "Immunohistochemistry-Paraffin: BAF57 Antibody - BSA Free [NBP1-90012]")
![Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012]](https://resources.rndsystems.com/images/products/BAF57-Antibody-Immunohistochemistry-Paraffin-NBP1-90012-img0010.jpg "Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012]")
Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012]
Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012] - Staining of human endometrium shows high expression.![Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012]](https://resources.rndsystems.com/images/products/BAF57-Antibody-Immunohistochemistry-Paraffin-NBP1-90012-img0011.jpg "Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012]")
Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012]
Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012] - Staining of human liver shows low expression as expected.![Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012]](https://resources.rndsystems.com/images/products/BAF57-Antibody-Immunohistochemistry-Paraffin-NBP1-90012-img0001.jpg "Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012]")
Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012]
Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-90012] - Staining of human stomach shows strong nuclear positivity in glandular cells.![BAF57 Antibody - BSA Free Western Blot: BAF57 Antibody - BSA Free [NBP1-90012]](https://resources.rndsystems.com/images/products/nbp1-90012_rabbit-polyclonal-baf57-antibody-15520258544784.jpg "Western Blot: BAF57 Antibody - BSA Free [NBP1-90012]")
Western Blot: BAF57 Antibody - BSA Free [NBP1-90012]
Analysis in human cell line MOLT-4.![Western Blot: BAF57 Antibody [NBP1-90012]](https://resources.rndsystems.com/images/products/BAF57-Antibody-Western-Blot-NBP1-90012-img0008.jpg "Western Blot: BAF57 Antibody [NBP1-90012]")
Western Blot: BAF57 Antibody [NBP1-90012]
Western Blot: BAF57 Antibody [NBP1-90012] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.![BAF57 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: BAF57 Antibody - BSA Free [NBP1-90012]](https://resources.rndsystems.com/images/products/nbp1-90012_rabbit-polyclonal-baf57-antibody-25620259103176.jpg "Chromatin Immunoprecipitation-exo-Seq: BAF57 Antibody - BSA Free [NBP1-90012]")
Chromatin Immunoprecipitation-exo-Seq: BAF57 Antibody - BSA Free [NBP1-90012]
ChIP-Exo-Seq composite graph for Anti-SMARCE1 (NBP1-90012) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for BAF57 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BAF57
Alternate Names
BAF57BRG1-associated factor 57, chromatin remodeling complex BRG1-associated factor 57, subfamily e, member 1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily E member 1
Entrez Gene IDs
6605 (Human)
Gene Symbol
SMARCE1
UniProt
Additional BAF57 Products
Product Documents for BAF57 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BAF57 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for BAF57 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for BAF57 Antibody - BSA Free
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Q: Can you tell me how baf 57 stains in normal breast tissue? and provide reference/pictures?
A: You can find images using this antibody to stain normal breast tissue here on Human Protein Atlas as this direct antibody was used for those staining images (https://www.proteinatlas.org/ENSG00000073584-SMARCE1/tissue/breast).
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