BATF Antibody (8A12) - Azide and BSA Free
Novus Biologicals | Catalog # H00010538-M01
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 8A12
Format
Azide and BSA Free
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Product Specifications
Immunogen
BATF (NP_006390, 34 a.a. ~ 125 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EKNRIAAQKSRQRQTQKADTLHLESEDLEKQNAALRKEIKQLTEELKYFTSVLNSHEPLCSVLAASTPSPPEVVYSAHAFHQPHVSSPRFQP
Specificity
BATF - basic leucine zipper transcription factor, ATF-like
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Novus Biologicals Mouse BATF Antibody (8A12) - Azide and BSA Free (H00010538-M01) is a monoclonal antibody validated for use in WB, ELISA, IP and ChIP. Anti-BATF Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for BATF Antibody (8A12) - Azide and BSA Free
Western Blot: BATF Antibody (8A12) [H00010538-M01]
Western Blot: BATF Antibody (8A12) [H00010538-M01] - BATF monoclonal antibody (M01), clone 8A12. Analysis of BATF expression in NIH/3T3.Western Blot: BATF Antibody (8A12) [H00010538-M01]
Western Blot: BATF Antibody (8A12) [H00010538-M01] - BATF monoclonal antibody (M01), clone 8A12. Analysis of BATF expression in PC-12.Western Blot: BATF Antibody (8A12) [H00010538-M01]
Western Blot: BATF Antibody (8A12) [H00010538-M01] - BATF monoclonal antibody (M01), clone 8A12 Analysis of BATF expression in Hela S3 NE.Western Blot: BATF Antibody (8A12) [H00010538-M01]
Western Blot: BATF Antibody (8A12) [H00010538-M01] - BATF monoclonal antibody (M01), clone 8A12. Analysis of BATF expression in Raw 264.7.Western Blot: BATF Antibody (8A12) [H00010538-M01]
Western Blot: BATF Antibody (8A12) [H00010538-M01] - Analysis of BATF expression in transfected 293T cell line by BATF monoclonal antibody (M01), clone 8A12.Lane 1: BATF transfected lysate(14.1 KDa).Lane 2: Non-transfected lysate.Immunoprecipitation: BATF Antibody (8A12) [H00010538-M01]
Immunoprecipitation: BATF Antibody (8A12) [H00010538-M01] - Analysis of BATF transfected lysate using anti-BATF monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BATF MaxPab rabbit polyclonal antibody.ELISA: BATF Antibody (8A12) [H00010538-M01]
ELISA: BATF Antibody (8A12) [H00010538-M01] - Detection limit for recombinant GST tagged BATF is approximately 1ng/ml as a capture antibody.Applications for BATF Antibody (8A12) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: BATF
Long Name
Activating Transcription Factor 1
Alternate Names
SFA2
Entrez Gene IDs
10538 (Human)
Gene Symbol
BATF
UniProt
Additional BATF Products
Product Documents for BATF Antibody (8A12) - Azide and BSA Free
Product Specific Notices for BATF Antibody (8A12) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for BATF Antibody (8A12) - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ChIP Protocol Video
- Chromatin Immunoprecipitation (ChIP) Protocol
- Chromatin Immunoprecipitation Protocol
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Immunoprecipitation Protocol
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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