BCAS1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-681

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BCAS1 Primary Antibodies »

Product Specifications

Immunogen

Reacts with residues 532-550 [DTNSLQNGDKLQKRPEKRQ] of human NABC1.

Specificity

Reacts with human NABC1. Reactivity to other species unknown.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BCAS1 Antibody - BSA Free

Western Blot: BCAS1 AntibodyBSA Free [NB100-681]

Western Blot: BCAS1 AntibodyBSA Free [NB100-681]

Western Blot: BCAS1 Antibody [NB100-681] - Western blot analysis of BCAS1 from 15 ug of MCF-7 cell lysate using NB100-681 at 1:1000 dilution. BCAS1 is a 60 kDa protein: however it forms homodimers, which are detected in MCF-7 cells.
Western Blot: BCAS1 AntibodyBSA Free [NB100-681]

Western Blot: BCAS1 AntibodyBSA Free [NB100-681]

Western Blot: BCAS1 Antibody [NB100-681] - Detection of NABC1 in MCF-7 whole cell lysate (15 ug) using a 1:1,000 dilution of NB 100-681.
Simple Western: BCAS1 AntibodyBSA Free [NB100-681]

Simple Western: BCAS1 AntibodyBSA Free [NB100-681]

Simple Western: BCAS1 Antibody [NB100-681] - Simple Western lane view shows a specific band for BCAS1 in 0.05 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for BCAS1 Antibody - BSA Free

Application
Recommended Usage

Simple Western

1:5000

Western Blot

1:100-1:2000
Application Notes
This antibody may be used for Western blot analysis where 2 bands are seen at ~120 and ~80 kDa. NABC1 is a 60 kDa protein; however it forms dimers, which are readily detectable in MCF-7 cells. No other applications have been tested. Suggested Working Dilutions:* Western blot - 1:500 to 1:1,000 *The investigator should determine the optimal dilution for a specific application.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in MCF-7 lysate 0.05 mg/mL, separated by Size, antibody dilution of 1:5000, apparent MW was 57 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BCAS1

A novel gene, Novel Amplified in Breast Cancer-1 (NABC1) is implicated in breast cancer development. Breast tumor lines that harbor amplification of the cancer-related amplicon 20q13.2 display high levels of the NABC1 protein compared to normal human fibroblasts or a breast cancer line that does not overexpress the NABC1 mRNA.

Alternate Names

breast carcinoma amplified sequence 1, breast carcinoma-amplified sequence 1, NABC1AIBC1Amplified and overexpressed in breast cancer, Novel amplified in breast cancer 1

Entrez Gene IDs

8537 (Human)

Gene Symbol

BCAS1

Additional BCAS1 Products

Product Documents for BCAS1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BCAS1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BCAS1 Antibody - BSA Free

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