BCAT1 Antibody (1F8) - Azide and BSA Free

Novus Biologicals | Catalog # H00000586-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F8

Format

Azide and BSA Free
View all BCAT1 Primary Antibodies »

Product Specifications

Immunogen

BCAT1 (AAH33864, 1 a.a. ~ 320 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDCSNGCSAECTGEGGSKEVVGTFKAKDLIVTPATILKEKPDPNNLVFGTVFTDHMLTVEWSSEFGWEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFRGVDNKIRLFQPNLNMDRMYRSAVRATLPVFDKEELLECIQQLVKLDQEWVPYSTSASLYIRPTFIGTEPSLGVKKPTKALLFVLLSPVGPYFSSGTFNPVSLWANPKYVRAWKGGTGDCKMGGNYGSSLFAQCEAVDNGCQQVLWLYGEDHQITEVGTMNLFLYWINEDGEEELATPPLDGIILPGVTRRCILDLAHQWDTELSLFSINLPDFLQFIYF

Specificity

BCAT1 - branched chain aminotransferase 1, cytosolic (1F8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for BCAT1 Antibody (1F8) - Azide and BSA Free

Western Blot: BCAT1 Antibody (1F8) [H00000586-M02]

Western Blot: BCAT1 Antibody (1F8) [H00000586-M02]

Western Blot: BCAT1 Antibody (1F8) [H00000586-M02] - BCAT1 monoclonal antibody (M02), clone 1F8. Analysis of BCAT1 expression in human kidney.
Western Blot: BCAT1 Antibody (1F8) [H00000586-M02]

Western Blot: BCAT1 Antibody (1F8) [H00000586-M02]

Western Blot: BCAT1 Antibody (1F8) [H00000586-M02] - BCAT1 monoclonal antibody (M02), clone 1F8. Analysis of BCAT1 expression in MCF-7.
Sandwich ELISA: BCAT1 Antibody (1F8) [H00000586-M02] - Detection limit for recombinant GST tagged BCAT1 is 1 ng/ml as a capture antibody.

Applications for BCAT1 Antibody (1F8) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BCAT1

This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. [provided by RefSeq]

Long Name

Branched Chain Amino Acid Transaminase 1

Alternate Names

BCAT-1, BCATC, BCT1, EC 2.6.1.42, ECA39, PNAS121, PP18

Entrez Gene IDs

586 (Human)

Gene Symbol

BCAT1

Additional BCAT1 Products

Product Documents for BCAT1 Antibody (1F8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for BCAT1 Antibody (1F8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BCAT1 Antibody (1F8) - Azide and BSA Free

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