Bcl-6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-76541

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Bcl-6 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EHVVDTCRKFIKASEAEMVSAIKPPREEFLNSRMLMPQDIMAYRGREVVENNLPLRSAPGCESRAFAPSLYSGLSTPPASYSMYSHLPVSSLLFSDEEFRDVR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bcl-6 Antibody - BSA Free

Western Blot: Bcl-6 Antibody [NBP2-76541]

Western Blot: Bcl-6 Antibody [NBP2-76541]

Western Blot: Bcl-6 Antibody [NBP2-76541] - Analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: Bcl-6 Antibody [NBP2-76541]

Immunocytochemistry/ Immunofluorescence: Bcl-6 Antibody [NBP2-76541]

Immunocytochemistry/Immunofluorescence: Bcl-6 Antibody [NBP2-76541] - Staining of human cell line RT4 shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.
Bcl-6 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: Bcl-6 Antibody - BSA Free [NBP2-76541]

Chromatin Immunoprecipitation-exo-Seq: Bcl-6 Antibody - BSA Free [NBP2-76541]

ChIP-Exo-Seq composite graph for Anti-BCL6 (NBP2-76541) tested in HepG2 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for Bcl-6 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bcl-6

Bcl6 reacts with Bcl6 gene product in follicular lymphomas, diffuse large B cell lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant Hodgkin's disease.

Long Name

B Cell Lymphoma/Leukemia 6

Alternate Names

Bcl-5, Bcl6, LAZ3, ZBTB27, ZNF51

Gene Symbol

BCL6

Additional Bcl-6 Products

Product Documents for Bcl-6 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Bcl-6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail