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BDY FL VH032 | Fluorescent Probes
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Description: High-affinity VHL fluorescent probe for TR-FRET and FP assays

Chemical Name: (2S,4R)-1-((S)-2-(tert-Butyl)-21-(5,5-difluoro-7,9-dimethyl-5H-5λ4,6λ4-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-3-yl)-4,19-dioxo-6,9,12,15-tetraoxa-3,18-diazahenicosanoyl)-4-hydroxy-N-(4-(4-methylthiazol-5-yl)benzyl)pyrrolidine-2-carboxamide

Purity: ≥95%

Product Details

Biological Activity

BDY FL VH032 is a high-affinity VHL fluorescent probe (Kd = 3.01 nM) with application in time-resolved fluorescence resonance energy-transfer (TR-FRET) assays for high-throughput identification and characterization of VHL ligands. BDY FL VH032 consists of a derivative of von Hippel-Lindau (VHL) ligand VH 032 (Cat. No. 6911) and a fluorophore BDY FL (Cat. No. 5465) (with excitation peak at 504 nm and emission peak at 520 nm), joined by a polyethylene glycol (PEG) 4 linker. The BDY FL VH032 VHL TR-FRET signal is stable through the 90 - 300 min incubation time, and the TR-FRET binding assay is sensitive, selective and resistant to assay interference. BDY FL VH032 also exhibits high VHL affinity in a fluorescence polarization (FP) assay. Excitation maximum ~ 504 nm; emission maximum ~ 520 nm.

Optical Data for BDY FL VH032

BDY FL VH032 Dye Spectra
Emission Color Green
λabs 502 nm
λem 510 nm
Closest Laser line 488 nm
Application TR-FRET and FP assays

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Technical Data

Soluble to 10 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from St. Jude Children's Research Hospital

Product Datasheets

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