Beclin 1 [p Ser234] Antibody - Azide Free

Novus Biologicals | Catalog # NBP2-29654

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Primate (100%), Rat (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all Beclin 1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH. Accession # Q14457. Accession # Q14457

Reactivity Notes

The antibody has been directly tested for reactivity in Western blots with recombinant HeLa and 293T cells. It is anticipated that the antibody will react with bovine, canine, mouse, non-human primate, rat, and sheep tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Modification

p Ser234

Specificity

Specific for endogenous levels of the ~60 kDa beclin 1 protein phosphorylated at Ser234. Immunolabeling of the beclin 1 band is decreased in the beclin 1 alanine substitution mutant.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Beclin 1 [p Ser234] Antibody - Azide Free

Western Blot: Beclin 1/ATG6 [p Ser234] Antibody [NBP2-29654] - Western Blot: Beclin 1/ATG6 [p Ser234] Antibody [NBP2-29654] - Beclin 1 [p Ser234] Antibody [NBP2-29654] - flag-beclin-1 WT immunoprecipitates expressed in 293T cells showing specific immunolabeling of beclin-1 when phosphorylated at S234 (WT). This immunolabeling is decreased in the beclin-1 double alanine substitution mutant (S234A, S295A).

Applications for Beclin 1 [p Ser234] Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:250
Application Notes
Immunolabeling of the beclin-1 band is decreased in the beclin-1 alanine substitution mutant. Additional bands may be observed in non-immunoprecipitated lysates.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Beclin 1

Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999). Beclin-1 has been shown to interact with 14-3-3 proteins through Akt-mediated phosphorylation at Ser234 (Wang et al., 2012).

Long Name

Beclin1, Autophagy-related

Alternate Names

ATG6, BECN1, GT197, VPS30

Entrez Gene IDs

8678 (Human)

Gene Symbol

BECN1

UniProt

Q14457 (Human)

Additional Beclin 1 Products

Product Documents for Beclin 1 [p Ser234] Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for Beclin 1 [p Ser234] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy

Citations for Beclin 1 [p Ser234] Antibody - Azide Free

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FAQs

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Associated Pathways

Akt Signaling Pathway Akt Signaling Pathway Thumbnail