Human Beclin 1 ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP2-69959
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Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 uL)
Sensitivity
0.10 ng/mL
Assay Range
0.16 - 10 ng/mL
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Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Species
Human
Specificity
This kit recognizes Human BECN1 in samples. No significant cross-reactivity or interference between Human BECN1 and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) CV% 5%
Inter-Assay Precision (Precision between assays) CV% 5.15%
Recovery for Human Beclin 1 ELISA Kit (Colorimetric)
Recovery
93-111%
Linearity
Scientific Data Images for Human Beclin 1 ELISA Kit (Colorimetric)
ELISA: Human Beclin 1 ELISA Kit (Colorimetric) [NBP2-69959] -
ELISA: Human Beclin 1 ELISA Kit (Colorimetric) [NBP2-69959] - Standard Curve ReferenceKit Contents for Human Beclin 1 ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Beclin 1
Beclin-1 encodes an evolutionarily conserved 60kDa coiled-coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication.
Expression of the Beclin1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin-1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.
Long Name
Beclin1, Autophagy-related
Alternate Names
ATG6, BECN1, GT197, VPS30
Gene Symbol
BECN1
Additional Beclin 1 Products
Product Documents for Human Beclin 1 ELISA Kit (Colorimetric)
Certificate of Analysis
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Product Specific Notices for Human Beclin 1 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
