beta 2-Microglobulin Antibody (B2M-01) [HRP]

Novus Biologicals | Catalog # NBP1-44520

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Bovine (Negative), Canine (Negative), Chicken (Negative), Mouse (Negative), Rabbit (Negative)

Cited:

Human

Applications

Validated:

Western Blot, ELISA

Cited:

ELISA

Label

HRP

Antibody Source

Monoclonal Mouse IgG2A Clone # B2M-01
View all beta 2-Microglobulin Primary Antibodies »

Product Specifications

Immunogen

Purified human beta 2 Microglobulin (NM_004048.2).

Reactivity Notes

Weak positive staining was observed in peripheral blood lymphocytes of the monkey Cercopithecus aethiops.

Specificity

The antibody B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Store in the dark. Avoid prolonged exposure to light.

Scientific Data Images for beta 2-Microglobulin Antibody (B2M-01) [HRP]

Western Blot: beta 2-Microglobulin Antibody (B2M-01) [HRP] [NBP1-44520] - Analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human beta2-microglobulin (B2M-01). Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line
ELISA: beta 2-Microglobulin Antibody (B2M-01) [HRP] [NBP1-44520] - Human serum using anti-HLA-G (capture) and anti-beta2 microglobulin HRP (detection).

Applications for beta 2-Microglobulin Antibody (B2M-01) [HRP]

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Preservative

0.01% Thimerosal

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: beta 2-Microglobulin

Beta 2 Microglobulin non covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Alternate Names

B2M, beta 2Microglobulin

Gene Symbol

B2M

Additional beta 2-Microglobulin Products

Product Documents for beta 2-Microglobulin Antibody (B2M-01) [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for beta 2-Microglobulin Antibody (B2M-01) [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Cancer Biomarkers

Citations for beta 2-Microglobulin Antibody (B2M-01) [HRP]

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